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Visualization of the Cellular Uptake and Trafficking of DNA Origami Nanostructures in Cancer Cells.
Wang, Pengfei; Rahman, Mohammad Aminur; Zhao, Zhixiang; Weiss, Kristin; Zhang, Chao; Chen, Zhengjia; Hurwitz, Selwyn J; Chen, Zhuo G; Shin, Dong M; Ke, Yonggang.
Afiliación
  • Wang P; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Emory University School of Medicine , Atlanta, Georgia 30322, United States.
  • Rahman MA; Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine , Atlanta, Georgia 30322, United States.
  • Zhao Z; Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine , Atlanta, Georgia 30322, United States.
  • Weiss K; Department of Dermatology, Xiangya Hospital, Central South University , Changsha, Hunan 410083, China.
  • Zhang C; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Emory University School of Medicine , Atlanta, Georgia 30322, United States.
  • Chen Z; Department of Biostatistics and Bioinformatics Shared Resource, Winship Cancer Institute, Emory University Rollins School of Public Health , Atlanta, Georgia 30322, United States.
  • Hurwitz SJ; Department of Biostatistics and Bioinformatics Shared Resource, Winship Cancer Institute, Emory University Rollins School of Public Health , Atlanta, Georgia 30322, United States.
  • Chen ZG; Department of Pediatrics, Emory University School of Medicine , Atlanta, Georgia 30322, United States.
  • Shin DM; Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine , Atlanta, Georgia 30322, United States.
  • Ke Y; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Emory University School of Medicine , Atlanta, Georgia 30322, United States.
J Am Chem Soc ; 140(7): 2478-2484, 2018 02 21.
Article en En | MEDLINE | ID: mdl-29406750
DNA origami is a promising molecular delivery system for a variety of therapeutic applications including cancer therapy, given its capability to fabricate homogeneous nanostructures whose physicochemical properties (size, shape, surface chemistry) can be precisely tailored. However, the correlation between DNA-origami design and internalization efficiency in different cancer cell lines remains elusive. We investigated the cellular uptake of four DNA-origami nanostructures (DONs) with programmed sizes and shapes in multiple human cancer cell lines. The cellular uptake efficiency of DONs was influenced by size, shape, and cell line. Scavenger receptors were responsible for the internalization of DONs into cancer cells. We observed distinct stages of the internalization process of a gold nanoparticle (AuNP)-tagged rod-shape DON, using high-resolution transmission electron microscopy. This study provides detailed understanding of cellular uptake and intracellular trafficking of DONs in cancer cells, and offers new insights for future optimization of DON-based drug delivery systems for cancer treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Nanopartículas del Metal / Oro Límite: Humans Idioma: En Revista: J Am Chem Soc Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Nanopartículas del Metal / Oro Límite: Humans Idioma: En Revista: J Am Chem Soc Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos