Your browser doesn't support javascript.
loading
CD1b Tetramers Identify T Cells that Recognize Natural and Synthetic Diacylated Sulfoglycolipids from Mycobacterium tuberculosis.
James, Charlotte A; Yu, Krystle K Q; Gilleron, Martine; Prandi, Jacques; Yedulla, Vijayendar R; Moleda, Zuzanna Z; Diamanti, Eleonora; Khan, Momin; Aggarwal, Varinder K; Reijneveld, Josephine F; Reinink, Peter; Lenz, Stefanie; Emerson, Ryan O; Scriba, Thomas J; Souter, Michael N T; Godfrey, Dale I; Pellicci, Daniel G; Moody, D Branch; Minnaard, Adriaan J; Seshadri, Chetan; Van Rhijn, Ildiko.
Afiliación
  • James CA; Department of Medicine, University of Washington Medical Center, 750 Republican Street, Suite E663, Seattle, WA 98115, USA; Department of Pathology, Molecular Medicine and Mechanisms of Disease Program, University of Washington, Seattle, WA 98195, USA.
  • Yu KKQ; Department of Medicine, University of Washington Medical Center, 750 Republican Street, Suite E663, Seattle, WA 98115, USA.
  • Gilleron M; Institut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France.
  • Prandi J; Institut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France.
  • Yedulla VR; Stratingh Institute for Chemistry, University of Groningen, 9747AG Groningen, the Netherlands.
  • Moleda ZZ; Stratingh Institute for Chemistry, University of Groningen, 9747AG Groningen, the Netherlands.
  • Diamanti E; School of Chemistry, University of Bristol, Bristol BS8 1TS, UK.
  • Khan M; School of Chemistry, University of Bristol, Bristol BS8 1TS, UK.
  • Aggarwal VK; School of Chemistry, University of Bristol, Bristol BS8 1TS, UK.
  • Reijneveld JF; Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584CL Utrecht, the Netherlands.
  • Reinink P; Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584CL Utrecht, the Netherlands.
  • Lenz S; Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584CL Utrecht, the Netherlands.
  • Emerson RO; Adaptive Biotechnologies, Seattle, WA 98102, USA.
  • Scriba TJ; South African Tuberculosis Vaccine Initiative and Institute of Infectious Disease and Molecular Medicine, Division of Immunology, Department of Pathology, University of Cape Town, Cape Town 7935, South Africa.
  • Souter MNT; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, VIC 3000, Australia; Australian Research Council Centre of Excellence for Advanced Molecular Imaging, University of Melbourne, Melbourne, VIC 3010, Australia.
  • Godfrey DI; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, VIC 3000, Australia; Australian Research Council Centre of Excellence for Advanced Molecular Imaging, University of Melbourne, Melbourne, VIC 3010, Australia.
  • Pellicci DG; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, VIC 3000, Australia; Australian Research Council Centre of Excellence for Advanced Molecular Imaging, University of Melbourne, Melbourne, VIC 3010, Australia.
  • Moody DB; Department of Rheumatology, Allergy & Immunology, Brigham and Women's Hospital, 60 Fenwood Road, Room 6006V, Boston, MA 02115, USA.
  • Minnaard AJ; Stratingh Institute for Chemistry, University of Groningen, 9747AG Groningen, the Netherlands.
  • Seshadri C; Department of Medicine, University of Washington Medical Center, 750 Republican Street, Suite E663, Seattle, WA 98115, USA. Electronic address: seshadri@uw.edu.
  • Van Rhijn I; Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584CL Utrecht, the Netherlands; Department of Rheumatology, Allergy & Immunology, Brigham and Women's Hospital, 60 Fenwood Road, Room 6006V, Boston, MA 02115, USA. Electronic address: i.vanrhij
Cell Chem Biol ; 25(4): 392-402.e14, 2018 04 19.
Article en En | MEDLINE | ID: mdl-29398561
Mycobacterial cell wall lipids bind the conserved CD1 family of antigen-presenting molecules and activate T cells via their T cell receptors (TCRs). Sulfoglycolipids (SGLs) are uniquely synthesized by Mycobacterium tuberculosis, but tools to study SGL-specific T cells in humans are lacking. We designed a novel hybrid synthesis of a naturally occurring SGL, generated CD1b tetramers loaded with natural or synthetic SGL analogs, and studied the molecular requirements for TCR binding and T cell activation. Two T cell lines derived using natural SGLs are activated by synthetic analogs independently of lipid chain length and hydroxylation, but differentially by saturation status. By contrast, two T cell lines derived using an unsaturated SGL synthetic analog were not activated by the natural antigen. Our data provide a bioequivalence hierarchy of synthetic SGL analogs and SGL-loaded CD1b tetramers. These reagents can now be applied to large-scale translational studies investigating the diagnostic potential of SGL-specific T cell responses or SGL-based vaccines.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis / Glucolípidos / Activación de Linfocitos / Linfocitos T / Antígenos CD1 / Mycobacterium tuberculosis / Antígenos Bacterianos Límite: Humans Idioma: En Revista: Cell Chem Biol Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis / Glucolípidos / Activación de Linfocitos / Linfocitos T / Antígenos CD1 / Mycobacterium tuberculosis / Antígenos Bacterianos Límite: Humans Idioma: En Revista: Cell Chem Biol Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos