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Modulation of Host Learning in Aedes aegypti Mosquitoes.
Vinauger, Clément; Lahondère, Chloé; Wolff, Gabriella H; Locke, Lauren T; Liaw, Jessica E; Parrish, Jay Z; Akbari, Omar S; Dickinson, Michael H; Riffell, Jeffrey A.
Afiliación
  • Vinauger C; Department of Biology, University of Washington, Seattle, WA 98195, USA.
  • Lahondère C; Department of Biology, University of Washington, Seattle, WA 98195, USA.
  • Wolff GH; Department of Biology, University of Washington, Seattle, WA 98195, USA.
  • Locke LT; Department of Biology, University of Washington, Seattle, WA 98195, USA.
  • Liaw JE; Department of Biology, University of Washington, Seattle, WA 98195, USA.
  • Parrish JZ; Department of Biology, University of Washington, Seattle, WA 98195, USA.
  • Akbari OS; Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA.
  • Dickinson MH; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
  • Riffell JA; Department of Biology, University of Washington, Seattle, WA 98195, USA. Electronic address: jriffell@uw.edu.
Curr Biol ; 28(3): 333-344.e8, 2018 02 05.
Article en En | MEDLINE | ID: mdl-29395917
How mosquitoes determine which individuals to bite has important epidemiological consequences. This choice is not random; most mosquitoes specialize in one or a few vertebrate host species, and some individuals in a host population are preferred over others. Mosquitoes will also blood feed from other hosts when their preferred is no longer abundant, but the mechanisms mediating these shifts between hosts, and preferences for certain individuals within a host species, remain unclear. Here, we show that olfactory learning may contribute to Aedes aegypti mosquito biting preferences and host shifts. Training and testing to scents of humans and other host species showed that mosquitoes can aversively learn the scent of specific humans and single odorants and learn to avoid the scent of rats (but not chickens). Using pharmacological interventions, RNAi, and CRISPR gene editing, we found that modification of the dopamine-1 receptor suppressed their learning abilities. We further show through combined electrophysiological and behavioral recordings from tethered flying mosquitoes that these odors evoke changes in both behavior and antennal lobe (AL) neuronal responses and that dopamine strongly modulates odor-evoked responses in AL neurons. Not only do these results provide direct experimental evidence that olfactory learning in mosquitoes can play an epidemiological role, but collectively, they also provide neuroanatomical and functional demonstration of the role of dopamine in mediating this learning-induced plasticity, for the first time in a disease vector insect.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dopamina / Aedes / Percepción Olfatoria / Odorantes Límite: Animals / Female / Humans Idioma: En Revista: Curr Biol Asunto de la revista: BIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dopamina / Aedes / Percepción Olfatoria / Odorantes Límite: Animals / Female / Humans Idioma: En Revista: Curr Biol Asunto de la revista: BIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido