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T-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1.
Maiques, Oscar; Barceló, Carla; Panosa, Anaïs; Pijuan, Jordi; Orgaz, Jose L; Rodriguez-Hernandez, Irene; Matas-Nadal, Clara; Tell, Gemma; Vilella, Ramón; Fabra, Angels; Puig, Susana; Sanz-Moreno, Victoria; Matias-Guiu, Xavier; Canti, Carles; Herreros, Judit; Marti, Rosa M; Macià, Anna.
Afiliación
  • Maiques O; University of Lleida, IRBLleida, Lleida, Spain.
  • Barceló C; University of Lleida, IRBLleida, Lleida, Spain.
  • Panosa A; University of Lleida, IRBLleida, Lleida, Spain.
  • Pijuan J; University of Lleida, IRBLleida, Lleida, Spain.
  • Orgaz JL; Tumour Plasticity Laboratory, Randall Division of Cell and Molecular Biophysics, New Hunt's House, King's College London, London, UK.
  • Rodriguez-Hernandez I; Tumour Plasticity Laboratory, Randall Division of Cell and Molecular Biophysics, New Hunt's House, King's College London, London, UK.
  • Matas-Nadal C; Department of Dermatology, Hospital Universitari Arnau de Vilanova, University of Lleida, IRBLleida, Lleida, Spain.
  • Tell G; Melanoma Unit, Department of Dermatology, Hospital Clínic de Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), University of Barcelona, Barcelona, Spain.
  • Vilella R; Centre of Biomedical Research on Rare Diseases (CIBERER), Instituto de Salud Carlos III (ISCIII), Madrid, Spain.
  • Fabra A; Department of Immunology, Hospital Clínic, Barcelona, Spain.
  • Puig S; Molecular Oncology, Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain.
  • Sanz-Moreno V; Melanoma Unit, Department of Dermatology, Hospital Clínic de Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), University of Barcelona, Barcelona, Spain.
  • Matias-Guiu X; Centre of Biomedical Research on Rare Diseases (CIBERER), Instituto de Salud Carlos III (ISCIII), Madrid, Spain.
  • Canti C; Tumour Plasticity Laboratory, Randall Division of Cell and Molecular Biophysics, New Hunt's House, King's College London, London, UK.
  • Herreros J; Department of Pathology and Molecular Genetics, Hospital Universitari Arnau de Vilanova, University of Lleida, IRBLleida, Lleida, Spain.
  • Marti RM; Centre of Biomedical Research on Cancer (CIBERONC), Instituto de Salud Carlos III (ISCIII), Madrid, Spain.
  • Macià A; University of Lleida, IRBLleida, Lleida, Spain.
Pigment Cell Melanoma Res ; 31(4): 484-495, 2018 07.
Article en En | MEDLINE | ID: mdl-29385656
Melanoma is a malignant tumor derived from melanocytes. Once disseminated, it is usually highly resistant to chemotherapy and is associated with poor prognosis. We have recently reported that T-type calcium channels (TTCCs) are overexpressed in melanoma cells and play an important role in melanoma progression. Importantly, TTCC pharmacological blockers reduce proliferation and deregulate autophagy leading to apoptosis. Here, we analyze the role of autophagy during migration/invasion of melanoma cells. TTCC Cav3.1 and LC3-II proteins are highly expressed in BRAFV600E compared with NRAS mutant melanomas, both in cell lines and biopsies. Chloroquine, pharmacological blockade, or gene silencing of TTCCs inhibit the autophagic flux and impair the migration and invasion capabilities, specifically in BRAFV600E melanoma cells. Snail1 plays an important role in motility and invasion of melanoma cells. We show that Snail1 is strongly expressed in BRAFV600E melanoma cells and patient biopsies, and its expression decreases when autophagy is blocked. These results demonstrate a role of Snail1 during BRAFV600E melanoma progression and strongly suggest that targeting macroautophagy and, particularly TTCCs, might be a good therapeutic strategy to inhibit metastasis of the most common melanoma type (BRAFV600E).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Movimiento Celular / Mutación Missense / Canales de Calcio Tipo T / Proteínas Proto-Oncogénicas B-raf / Factores de Transcripción de la Familia Snail / Melanoma / Proteínas Asociadas a Microtúbulos Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Pigment Cell Melanoma Res Asunto de la revista: NEOPLASIAS Año: 2018 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Movimiento Celular / Mutación Missense / Canales de Calcio Tipo T / Proteínas Proto-Oncogénicas B-raf / Factores de Transcripción de la Familia Snail / Melanoma / Proteínas Asociadas a Microtúbulos Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Pigment Cell Melanoma Res Asunto de la revista: NEOPLASIAS Año: 2018 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido