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Measurement of Typhi Vi antibodies can be used to assess adaptive immunity in patients with immunodeficiency.
Evans, C; Bateman, E; Steven, R; Ponsford, M; Cullinane, A; Shenton, C; Duthie, G; Conlon, C; Jolles, S; Huissoon, A P; Longhurst, H J; Rahman, T; Scott, C; Wallis, G; Harding, S; Parker, A R; Ferry, B L.
Afiliación
  • Evans C; Clinical Immunology Laboratory, Oxford University Hospitals Foundation Trust, Oxford, UK.
  • Bateman E; Clinical Immunology Laboratory, Oxford University Hospitals Foundation Trust, Oxford, UK.
  • Steven R; Immunodeficiency Centre for Wales, University Hospital of Wales, Cardiff, UK.
  • Ponsford M; Immunodeficiency Centre for Wales, University Hospital of Wales, Cardiff, UK.
  • Cullinane A; Clinical Immunology Laboratory, Oxford University Hospitals Foundation Trust, Oxford, UK.
  • Shenton C; Clinical Immunology Laboratory, Oxford University Hospitals Foundation Trust, Oxford, UK.
  • Duthie G; Infectious Disease Department, Oxford University Hospitals Foundation Trust, Oxford, UK.
  • Conlon C; Infectious Disease Department, Oxford University Hospitals Foundation Trust, Oxford, UK.
  • Jolles S; Immunodeficiency Centre for Wales, University Hospital of Wales, Cardiff, UK.
  • Huissoon AP; West Midlands Primary Immunodeficiency Centre, Birmingham Heartlands Hospital, Birmingham, UK.
  • Longhurst HJ; Department of Immunology, Barts Health NHS Trust, London, UK.
  • Rahman T; Department of Immunology, Barts Health NHS Trust, London, UK.
  • Scott C; Department of Immunology, Barts Health NHS Trust, London, UK.
  • Wallis G; Binding Site Group Limited, Birmingham, UK.
  • Harding S; Binding Site Group Limited, Birmingham, UK.
  • Parker AR; Binding Site Group Limited, Birmingham, UK.
  • Ferry BL; Clinical Immunology Laboratory, Oxford University Hospitals Foundation Trust, Oxford, UK.
Clin Exp Immunol ; 192(3): 292-301, 2018 06.
Article en En | MEDLINE | ID: mdl-29377063
Vaccine-specific antibody responses are essential in the diagnosis of antibody deficiencies. Responses to Pneumovax II are used to assess the response to polysaccharide antigens, but interpretation may be complicated. Typhim Vi® , a polysaccharide vaccine for Salmonella typhoid fever, may be an additional option for assessing humoral responses in patients suspected of having an immunodeficiency. Here we report a UK multi-centre study describing the analytical and clinical performance of a Typhi Vi immunoglobulin (Ig)G enzyme-linked immunosorbent assay (ELISA) calibrated to an affinity-purified Typhi Vi IgG preparation. Intra- and interassay imprecision was low and the assay was linear, between 7·4 and 574 U/ml (slope = 0·99-1·00; R2  > 0·99); 71% of blood donors had undetectable Typhi Vi IgG antibody concentrations. Of those with antibody concentrations  > 7·4 U/ml, the concentration range was 7·7-167 U/ml. In antibody-deficient patients receiving antibody replacement therapy the median Typhi Vi IgG antibody concentrations were  < 25 U/ml. In vaccinated normal healthy volunteers, the median concentration post-vaccination was 107 U/ml (range 31-542 U/ml). Eight of eight patients (100%) had post-vaccination concentration increases of at least threefold and six of eight (75%) of at least 10-fold. In an antibody-deficient population (n = 23), only 30% had post-vaccination concentration increases of at least threefold and 10% of at least 10-fold. The antibody responses to Pneumovax II and Typhim Vi® correlated. We conclude that IgG responses to Typhim Vi® vaccination can be measured using the VaccZyme Salmonella typhi Vi IgG ELISA, and that measurement of these antibodies maybe a useful additional test to accompany Pneumovax II responses for the assessment of antibody deficiencies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos Bacterianos / Inmunoglobulina G / Ensayo de Inmunoadsorción Enzimática / Vacunas Tifoides-Paratifoides / Inmunidad Adaptativa / Síndromes de Inmunodeficiencia / Anticuerpos Antibacterianos Tipo de estudio: Clinical_trials Límite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Clin Exp Immunol Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos Bacterianos / Inmunoglobulina G / Ensayo de Inmunoadsorción Enzimática / Vacunas Tifoides-Paratifoides / Inmunidad Adaptativa / Síndromes de Inmunodeficiencia / Anticuerpos Antibacterianos Tipo de estudio: Clinical_trials Límite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Clin Exp Immunol Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido