Antioxidative properties and structural features of atypical 2-Cys peroxiredoxin from Sebastes schlegelii.

Godahewa, G I; Perera, N C N; Nam, Bo-Hye; Lee, Jehee

Godahewa, G I; Perera, N C N; Nam, Bo-Hye; Lee, Jehee.

Afiliación

Godahewa GI; Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea.
Perera NCN; Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea.
Nam BH; Biotechnology Research Division, National Institute of Fisheries Science, 408-1 Sirang-ri, Gijang-up, Gijang-gun, Busan, 46083, Republic of Korea.
Lee J; Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea; Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea. Electronic address: jehee@jejunu.ac.kr.

Dev Comp Immunol ; 82: 152-164, 2018 05.

Article en En | MEDLINE | ID: mdl-29374514

RESUMEN

Atypical 2-Cys peroxiredoxin (Prx5) is an antioxidant protein that exerts its antioxidant function by detoxifying different reactive oxygen species (ROS). Here, we identified mitochondrial Prx5 from rockfish (SsPrx5) and described its specific structural and functional characteristics. The open reading frame (ORF) of SsPrx5 (570 bp) was translated into a 190-amino acid polypeptide that contained a mitochondrial targeting sequence (MTS), thioredoxin 2 domain, two Prx-specific signature motifs, and three conserved cysteine residues. Sequence comparison indicated that the SsPrx5 protein sequence shared greatest identity with teleost orthologs, where the phylogenetic results showed an evolutionary position within the fish Prx5. The coding sequence of SsPrx5 was scattered in six exons as found in other vertebrates. Additionally, the potent antioxidant functions of recombinantly expressed SsPrx5 protein was demonstrated by insulin reduction and extracellular H2O2 scavenging both in vitro and in vivo. Quantitative real time PCR (qPCR) detected ubiquitous mRNA expression of SsPrx5 in healthy rockfish tissues, with remarkable expression observed in gill, liver, and reproductive tissues. Prompt transcription of SsPrx5 was shown in the immune-stimulated gill and liver tissues against Streptococcus iniae and lipopolysaccharide injection. Taken together, present results suggest the indispensable role of SsPrx5 in the rockfish antioxidant defense system against oxidative stresses and its role in maintaining redox balance upon pathogen invasion.

Asunto(s)

Proteínas de Peces/genética; Peces/metabolismo; Branquias/metabolismo; Hígado/metabolismo; Mitocondrias/metabolismo; Peroxirredoxinas/genética; Infecciones Estreptocócicas/inmunología; Streptococcus iniae/inmunología; Animales; Antioxidantes; Clonación Molecular; Cisteína/genética; Proteínas de Peces/metabolismo; Insulina/metabolismo; Oxidación-Reducción; Estrés Oxidativo; Peroxirredoxinas/metabolismo; Filogenia; Especies Reactivas de Oxígeno/metabolismo; Relación Estructura-Actividad; Transcriptoma; Vertebrados

Palabras clave

Extracellular H(2)O(2) scavenging; Insulin reduction; Peroxiredoxin 5; mRNA expression

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estreptocócicas / Proteínas de Peces / Peroxirredoxinas / Peces / Streptococcus iniae / Branquias / Hígado / Mitocondrias Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Dev Comp Immunol Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos

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