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Effect of inoculum size on single-cell oil production from glucose and xylose using oleaginous yeast Lipomyces starkeyi.
Juanssilfero, Ario B; Kahar, Prihardi; Amza, Rezky L; Miyamoto, Nao; Otsuka, Hiromi; Matsumoto, Hana; Kihira, Chie; Thontowi, Ahmad; Ogino, Chiaki; Prasetya, Bambang; Kondo, Akihiko.
Afiliación
  • Juanssilfero AB; Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan; Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km 46, Cibinong, 16911 Bogor, West Java, Indonesia.
  • Kahar P; Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
  • Amza RL; Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
  • Miyamoto N; Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
  • Otsuka H; Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
  • Matsumoto H; Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
  • Kihira C; Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
  • Thontowi A; Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km 46, Cibinong, 16911 Bogor, West Java, Indonesia.
  • Yopi; Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km 46, Cibinong, 16911 Bogor, West Java, Indonesia.
  • Ogino C; Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan. Electronic address: ochiaki@port.kobe-u.ac.jp.
  • Prasetya B; National Standardization Agency of Indonesia (BSN), Gedung Badan Pengkajian Dan Penerapan Teknologi (BPPT), Jl. M.H. Thamrin No. 8, Jakarta 10340, Indonesia.
  • Kondo A; Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan; Graduate School of Science, Technology and Innovation (STIN), Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
J Biosci Bioeng ; 125(6): 695-702, 2018 Jun.
Article en En | MEDLINE | ID: mdl-29373308
Oleaginous microbes can convert substrates such as carbon dioxide, sugars, and organic acids to single-cell oils (SCOs). Among the oleaginous microorganisms, Lipomyces starkeyi is a particularly well-suited host given its impressive native abilities, including the capability to utilize a wide variety of carbon sources. In this work, the potential of L. starkeyi NBRC10381 to produce SCOs in a synthetically nitrogen-limited mineral medium (-NMM) was investigated by differing the inoculum size using glucose and/or xylose as a carbon source. Fermentation using glucose and xylose as mixed carbon sources generated the highest production of biomass at 40.8 g/L, and achieved a lipid content of 84.9% (w/w). When either glucose or xylose was used separately, the totals for achieved lipid content were 79.6% (w/w) and 85.1% (w/w), respectively. However, biomass production was higher for glucose than for xylose (30.3 vs. 28.7 g/L, respectively). This study describes the first simultaneous achievement of higher levels of cell mass and lipid production using glucose and/or xylose as the carbon sources in different inoculum sizes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Xilosa / Aceites / Lipomyces / Glucosa Idioma: En Revista: J Biosci Bioeng Asunto de la revista: ENGENHARIA BIOMEDICA / MICROBIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Japón
Texto completo
Añadir a Mi BVS
Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Xilosa / Aceites / Lipomyces / Glucosa Idioma: En Revista: J Biosci Bioeng Asunto de la revista: ENGENHARIA BIOMEDICA / MICROBIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Japón