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Surface IgM λ light chain is involved in the binding and infection of infectious bursal disease virus (IBDV) to DT40 cells.
Chi, Jiaqi; You, Leiming; Li, Peipei; Teng, Man; Zhang, Gaiping; Luo, Jun; Wang, Aiping.
Afiliación
  • Chi J; Department of Oncology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, 100078, People's Republic of China.
  • You L; School of Life Sciences, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.
  • Li P; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, No. 116 Huayuan Road, Zhengzhou, 450002, People's Republic of China.
  • Teng M; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, No. 116 Huayuan Road, Zhengzhou, 450002, People's Republic of China.
  • Zhang G; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, No. 116 Huayuan Road, Zhengzhou, 450002, People's Republic of China.
  • Luo J; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, No. 116 Huayuan Road, Zhengzhou, 450002, People's Republic of China. luojun593@aliyun.com.
  • Wang A; School of Life Sciences, Zhengzhou University, No. 100 Kexue Road, Zhengzhou, 450002, People's Republic of China. pingaw@126.com.
Virus Genes ; 54(2): 236-245, 2018 Apr.
Article en En | MEDLINE | ID: mdl-29372383
Infectious bursal disease virus (IBDV) is an important immunosuppressive virus in chickens. Surface immunoglobulin M (sIgM)-bearing B lymphocytes act as the major targets of IBDV in the bursa of Fabricius, and sIgM may function as one of the membrane binding sites responsible for IBDV infection. Recently, using the virus overlay protein binding assay, the chicken λ light chain of sIgM was identified to specifically interact with IBDV in a virulence-independent manner in vitro. To further investigate sIgM λ light chain-mediated IBDV binding and infection in pre-B cells, the cell line DT40, which is susceptible to both pathogenic and attenuated IBDV, was used. Based on the RNA interference strategy, the DT40 cell line whose λ light chain of sIgM was stably knocked down, herein termed DT40LKD, was generated by the genomic integration of a specific small hairpin RNA and a green fluorescence protein co-expression construct. Flow cytometry analysis indicated that the binding of IBDV to DT40LKD cells was significantly reduced due to the loss of sIgM λ light chain. In particular, reduced viral replication was observed in IBDV-incubated DT40LKD cells, and no viral release into cell culture medium was detected by the IBDV rapid diagnostic strips. In addition, the rescue of sIgM λ light chain expression restored viral binding and replication in DT40LKD cells. These results show that sIgM λ light chain appears to be beneficial for IBDV attachment and infection, suggesting that sIgM acts as a binding site involved in IBDV infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores Virales / Inmunoglobulina M / Linfocitos B / Cadenas Ligeras de Inmunoglobulina / Virus de la Enfermedad Infecciosa de la Bolsa / Acoplamiento Viral / Proteínas de la Membrana Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Virus Genes Asunto de la revista: BIOLOGIA MOLECULAR / VIROLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores Virales / Inmunoglobulina M / Linfocitos B / Cadenas Ligeras de Inmunoglobulina / Virus de la Enfermedad Infecciosa de la Bolsa / Acoplamiento Viral / Proteínas de la Membrana Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Virus Genes Asunto de la revista: BIOLOGIA MOLECULAR / VIROLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos