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SLC30A family expression in the pancreatic islets of humans and mice: cellular localization in the ß-cells.
Cai, Yimeng; Kirschke, Catherine P; Huang, Liping.
Afiliación
  • Cai Y; Graduate Group of Nutritional Biology, Department of Nutrition, University of California Davis, One Shields Ave, Davis, CA, 95616, USA.
  • Kirschke CP; Obesity and Metabolism Research Unit, USDA/ARS/Western Human Nutrition Research Center, 430 West Health Sciences Drive, Davis, CA, 95616, USA.
  • Huang L; Graduate Group of Nutritional Biology, Department of Nutrition, University of California Davis, One Shields Ave, Davis, CA, 95616, USA. liping.huang@ars.usda.gov.
J Mol Histol ; 49(2): 133-145, 2018 Apr.
Article en En | MEDLINE | ID: mdl-29372370
Zinc is a vital co-factor for insulin metabolism in the pancreatic ß-cell, involved in synthesis, maturation, and crystallization. Two families of zinc transporters, namely SLC30A (ZNT) and SLC39A (ZIP) are involved in maintaining cellular zinc homeostasis in mammalian cells. Single nuclear polymorphisms or mutations in zinc transporters have been associated with insulin resistance and risk of type 2 diabetes (T2D) in both humans and mice. Thus, mice can be useful for studying the underlying mechanisms of zinc-associated risk of T2D development. To determine potential differences in zinc transporter expression and cellular localization in the pancreatic ß-cells between humans and mice, we examined all members (ZNT1-10) of the ZNT family in pancreatic islets and in ß-cell lines derived from both species using immunohistochemistry and immunofluorescence microscopic analysis. We found that there were no substantial differences in the expression of nine ZNT proteins in the human and mouse islets and ß-cells with exception of ZNT3, which was only detected in human ß-cells, but not in mouse ß-cells. Moreover, we found that ZNT2 was localized on the cell surface of both human and mouse ß-cells, suggesting a role of ZNT2 in direct export of zinc out of the ß-cell. Together, our study suggests functional conservations of the ZNT proteins between humans and mice. We believe that our results are of interest for future studies in the association of zinc metabolism with risk of T2D in humans using mouse models.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Islotes Pancreáticos / Proteínas de Transporte de Catión / Células Secretoras de Insulina Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: J Mol Histol Asunto de la revista: HISTOCITOQUIMICA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Islotes Pancreáticos / Proteínas de Transporte de Catión / Células Secretoras de Insulina Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: J Mol Histol Asunto de la revista: HISTOCITOQUIMICA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos