Influence of antibody and complement components on phagocytosis and chemiluminescence of macrophages.
Immunobiology
; 170(5): 390-401, 1985 Dec.
Article
en En
| MEDLINE
| ID: mdl-2936676
Macrophages are known to release reactive oxygen species (O2-, 1O2, H2O2, OH.) in response to various membrane stimuli. However, our studies show that phagocytic stimulation of macrophages is not necessarily accompanied by a stimulation of the oxidative burst. Whereas IgG-opsonized erythrocytes were capable to induce phagocytosis and a chemiluminescence response, both being dependent on the number of IgG bound per erythrocyte, C3b-bearing erythrocytes were well ingested but failed to induce any chemiluminescence reaction. Furthermore, stimulation of macrophages, via the Fc-receptors, seems to alter their functional state in regard to the activation of a receptor, which enables them to recognize membrane lesions on the target erythrocyte. The presence of IgG and membrane lesions, e.g. the C5b-9-complex of complement, induced a marked increase in chemiluminescence compared with stimulation by IgG-bearing particles alone. The augmented response of macrophages was at least in part due to an additional release of H2O2, which was not liberated in response to IgG-bearing erythrocytes. This "lesion recognizing receptor" in the macrophage membrane could not be activated by stimulation of C3b-receptors, indicating its functional linkage to the Fc-receptors.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fagocitosis
/
Complemento C5
/
Complemento C9
/
Macrófagos
Límite:
Animals
Idioma:
En
Revista:
Immunobiology
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Países Bajos