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An OLTAM system for analysis of brown/beige fat thermogenic activity.
Kim, D-I; Liao, J; Emont, M P; Park, M-J; Jun, H; Ramakrishnan, S K; Lin, J D; Shah, Y M; Omary, M B; Wu, J.
Afiliación
  • Kim DI; Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
  • Liao J; Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
  • Emont MP; Department of Endocrinology and Metabolism, 2nd Xiangya Hospital, Central South University, Changsha, Hunan, China.
  • Park MJ; Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
  • Jun H; Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
  • Ramakrishnan SK; Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
  • Lin JD; Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
  • Shah YM; Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
  • Omary MB; Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
  • Wu J; Department of Cell & Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Int J Obes (Lond) ; 42(4): 939-945, 2018 04.
Article en En | MEDLINE | ID: mdl-29359735
BACKGROUND/OBJECTIVES: Thermogenic fat is present in humans and emerging evidence indicates that increasing the content and activity of these adipocytes may lead to weight loss and improved metabolic health. Multiple reporter systems have been developed to assay thermogenic fat activity based on the transcriptional and translational activation of Ucp1, the key molecule that mediates nonshivering thermogenesis. Our study aims to develop a much-needed tool to monitor thermogenic fat activity through a mechanism independent of Ucp1 regulation, therefore effectively assaying not only canonical ß-adrenergic activation but also various non-UCP1-mediated thermogenic pathways that have been increasingly appreciated. METHODS: We detected increased luciferase activity upon thermogenic activation in interscapular brown and inguinal subcutaneous fat in ODD-Luc mice, a hypoxia reporter mouse model. We then developed an OLTAM (ODD-Luc based Thermogenic Activity Measurement) system to assay thermogenic fat cell activity. RESULTS: In both primary murine and human adipocytes and an immortalized adipose cell line that were transduced with the OLTAM system, luciferase activity can be readily measured and visualized by bioluminescence imaging in response to a variety of stimuli, including UCP1-independent thermogenic signaling. This system can offer a convenient method to assay thermogenic activity for both basic and translational research. CONCLUSIONS: The OLTAM system offers a convenient way to measure the activation of thermogenic fat and presents opportunities to discover novel signaling pathways and unknown compounds targeting metabolically active adipocytes to counteract human obesity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tejido Adiposo Pardo / Termografía / Termogénesis / Tejido Adiposo Beige Límite: Adult / Animals / Female / Humans Idioma: En Revista: Int J Obes (Lond) Asunto de la revista: METABOLISMO Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tejido Adiposo Pardo / Termografía / Termogénesis / Tejido Adiposo Beige Límite: Adult / Animals / Female / Humans Idioma: En Revista: Int J Obes (Lond) Asunto de la revista: METABOLISMO Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido