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Enhanced reducing sugar production by saccharification of lignocellulosic biomass, Pennisetum species through cellulase from a newly isolated Aspergillus fumigatus.
Mohapatra, Sonali; Padhy, Sameer; Das Mohapatra, Pradeep K; Thatoi, H N.
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  • Mohapatra S; Department of Biotechnology, College of Engineering and Technology, Biju Pattnaik University of Technology, Bhubaneswar 751003, India.
  • Padhy S; Department of Biotechnology, College of Engineering and Technology, Biju Pattnaik University of Technology, Bhubaneswar 751003, India.
  • Das Mohapatra PK; Department of Microbiology, Vidyasagar University, Midnapore 721102, India. Electronic address: pkm@mail.vidyasagar.ac.in.
  • Thatoi HN; Department of Biotechnology, North Orissa University, Sriram Chandra vihar, Takatpur, Baripada 757003, Odisha, India.
Bioresour Technol ; 253: 262-272, 2018 Apr.
Article en En | MEDLINE | ID: mdl-29353755
A cellulose degrading fungus Aspergillus fumigatus (CWSF-7) isolated from decomposed lignocellulosic waste containing soil was found to produce high titer of cellulases. The optimum activity of CMCase and FPase were 1.9 U/mL and 0.9 U/mL respectively while the highest protein concentration was found to be 1.2 mg/mL. Saccharification of two Pennisetum grass varieties [dennanath (DG) and hybrid napier grass (HNG)] were optimized using partially purified CMCase and FPase in equal concentration, i.e. a ratios of 1:1 and further with addition of commercial xylanase using response surface methodology (RSM). The production of total reducing sugar (TRS) using isolated cellulase were 396.6 and 355.8 (mg/g), whereas further addition of xylanase had higher TRS titers of 478.7 and 483.3 (mg/g) for DG and HNG respectively as evident from HPLC analysis. Further, characterization of the enzyme saccharified DG and HNG by SEM and ATR-FTIR revealed efficient hydrolysis of cellulose and partially hydrolysis of hemicellulose.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus fumigatus / Pennisetum Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2018 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus fumigatus / Pennisetum Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2018 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido