Your browser doesn't support javascript.
loading
Simultaneous quantification of four antiretroviral drugs in breast milk samples from HIV-positive women by an ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method.
Ramírez-Ramírez, Alicia; Sánchez-Serrano, Elías; Loaiza-Flores, Giselle; Plazola-Camacho, Noemí; Rodríguez-Delgado, Rosa Georgina; Figueroa-Damián, Ricardo; Domínguez-Castro, Mauricio; López-Martínez, Margarita; Flores-García, Zayra; Hernández-Pineda, Jessica.
Afiliación
  • Ramírez-Ramírez A; Departement of Infectology and Immunology, National Institute of Perinatology, Mexico City, Mexico.
  • Sánchez-Serrano E; Chemistry Faculty, National Autonomous University of Mexico, Mexico City, Mexico.
  • Loaiza-Flores G; Chemistry Faculty, National Autonomous University of Mexico, Mexico City, Mexico.
  • Plazola-Camacho N; Departement of Infectology and Immunology, National Institute of Perinatology, Mexico City, Mexico.
  • Rodríguez-Delgado RG; Departement of Infectology and Immunology, National Institute of Perinatology, Mexico City, Mexico.
  • Figueroa-Damián R; Departement of Infectology and Immunology, National Institute of Perinatology, Mexico City, Mexico.
  • Domínguez-Castro M; Department of Physiology and Cellular Development, National Institute of Perinatology, Mexico City, Mexico.
  • López-Martínez M; Department of Physiology and Cellular Development, National Institute of Perinatology, Mexico City, Mexico.
  • Flores-García Z; Faculty of Higher Education, National Autonomous University of Mexico, Cuautitlan Izcalli, Mexico State, Mexico.
  • Hernández-Pineda J; Departement of Infectology and Immunology, National Institute of Perinatology, Mexico City, Mexico.
PLoS One ; 13(1): e0191236, 2018.
Article en En | MEDLINE | ID: mdl-29351333
The primary strategy to avoid mother-to-child transmission of human immunodeficiency virus (HIV) through breastfeeding is administration of highly active antiretroviral therapy (HAART) to HIV-positive pregnant women. Because significant changes in the pharmacokinetics of antiretroviral (ARV) drugs occur during pregnancy, quantifying HAART and the viral load in breast milk in this population is essential. Here, we developed an analytical assay for the simultaneous quantification of four ARV drugs in breast milk using ultra-performance liquid chromatography coupled to tandem mass spectrometry. We validated this method following Mexican and international guidelines. ARV drugs. We extracted the ARV drugs from 200 µL samples of breast milk and detected these drugs in a triple quadrupole mass spectrometer with positive electrospray ionization. The validated concentration ranges (ng/mL) for zidovudine, lamivudine, lopinavir, and ritonavir were 12.5-750, 50-2500, 100-5000 and 5 to 250, respectively. Additionally, the absolute recovery percentages (and matrix effects) were 91.4 (8.39), 88.78 (28.75), 91.38 (11.77) and 89.78 (12.37), respectively. We determined that ARV drugs are stable for 24 h at 8°C and 24°C for 15 days at -80°C. This methodology had the capacity for simultaneous detection; separation; and accurate, precise quantification of ARV drugs in human breast milk samples according to Mexican standard laws and United States Food and Drug Administration guidelines.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Complicaciones Infecciosas del Embarazo / Infecciones por VIH / Fármacos Anti-VIH / Terapia Antirretroviral Altamente Activa / Leche Humana Tipo de estudio: Guideline Límite: Adult / Female / Humans / Newborn / Pregnancy Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article País de afiliación: México Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Complicaciones Infecciosas del Embarazo / Infecciones por VIH / Fármacos Anti-VIH / Terapia Antirretroviral Altamente Activa / Leche Humana Tipo de estudio: Guideline Límite: Adult / Female / Humans / Newborn / Pregnancy Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article País de afiliación: México Pais de publicación: Estados Unidos