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Identification of Cyclic Dipeptides from Escherichia coli as New Antimicrobial Agents against Ralstonia Solanacearum.
Song, Shihao; Fu, Shuna; Sun, Xiuyun; Li, Peng; Wu, Ji'en; Dong, Tingyan; He, Fei; Deng, Yinyue.
Afiliación
  • Song S; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China. lklssh@stu.scau.edu.cn.
  • Fu S; Guangdong Innovative Research Team of Sociomicrobiology, College of Agriculture, South China Agricultural University, Guangzhou 510642, China. lklssh@stu.scau.edu.cn.
  • Sun X; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China. fu_shuna@163.com.
  • Li P; Guangdong Innovative Research Team of Sociomicrobiology, College of Agriculture, South China Agricultural University, Guangzhou 510642, China. fu_shuna@163.com.
  • Wu J; Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China. fu_shuna@163.com.
  • Dong T; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China. sunxiuyun1998@163.com.
  • He F; Guangdong Innovative Research Team of Sociomicrobiology, College of Agriculture, South China Agricultural University, Guangzhou 510642, China. sunxiuyun1998@163.com.
  • Deng Y; Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China. sunxiuyun1998@163.com.
Molecules ; 23(1)2018 Jan 19.
Article en En | MEDLINE | ID: mdl-29351264
Ralstonia solanacearum is a causative agent of bacterial wilt in many important crops throughout the world. How to control bacterial wilt caused by R. solanacearum is a major problem in agriculture. In this study, we aim to isolate the biocontrol agents that have high efficacy in the control of bacterial wilt. Three new bacterial strains with high antimicrobial activity against R. solanacearum GMI1000 were isolated and identified. Our results demonstrated that these bacteria could remarkably inhibit the disease index of host plant infected by R. solanacearum. It was indicated that strain GZ-34 (CCTCC No. M 2016353) showed an excellent protective effect to tomato under greenhouse conditions. Strain GZ-34 was characterized as Escherichia coli based on morphology, biochemistry, and 16S rRNA analysis. We identified that the main antimicrobial compounds produced by E. coli GZ-34 were cyclo(l-Pro-d-Ile) and cyclo(l-Pro-l-Phe) using electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) analysis. The two active compounds also interfered with the expression levels of some pathogenicity-contributors of R. solanacearum. Furthermore, cyclo(l-Pro-l-Phe) effectively inhibited spore formation of Magnaporthe grisea, which is a vital pathogenesis process of the fungal pathogen, suggesting cyclic dipeptides from E. coli are promising potential antimicrobial agents with broad-spectrum activity to kill pathogens or interfere with their pathogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos Cíclicos / Ralstonia solanacearum / Dipéptidos / Escherichia coli / Antiinfecciosos / Antibiosis Tipo de estudio: Diagnostic_studies Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos Cíclicos / Ralstonia solanacearum / Dipéptidos / Escherichia coli / Antiinfecciosos / Antibiosis Tipo de estudio: Diagnostic_studies Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza