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Quantitation of saxitoxin in human urine using immunocapture extraction and LC-MS.
Bragg, William A; Garrett, Alaine; Hamelin, Elizabeth I; Coleman, Rebecca M; Campbell, Katrina; Elliott, Christopher T; Johnson, Rudolph C.
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  • Bragg WA; Emergency Response Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control & Prevention, Atlanta, GA, 30341 USA.
  • Garrett A; ORISE Fellow, Centers for Disease Control & Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Atlanta, GA 30341 USA.
  • Hamelin EI; Emergency Response Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control & Prevention, Atlanta, GA, 30341 USA.
  • Coleman RM; ORISE Fellow, Centers for Disease Control & Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Atlanta, GA 30341 USA.
  • Campbell K; Institute for Global Food Security, Queen's University, David Keir Building, Belfast, Northern Ireland, UK.
  • Elliott CT; Institute for Global Food Security, Queen's University, David Keir Building, Belfast, Northern Ireland, UK.
  • Johnson RC; Emergency Response Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control & Prevention, Atlanta, GA, 30341 USA.
Bioanalysis ; 10(4): 229-239, 2018 Feb.
Article en En | MEDLINE | ID: mdl-29333869
AIM: An immunomagnetic capture protocol for use with LC-MS was developed for the quantitation of saxitoxin (STX) in human urine. MATERIALS & METHODS: This method uses monoclonal antibodies coupled to magnetic beads. STX was certified reference material grade from National Research Council, Canada. Analysis was carried out using LC-MS. RESULTS: With an extraction efficiency of 80%, accuracy and precision of 93.0-100.2% and 5.3-12.6%, respectively, and a dynamic range of 1.00-100 ng/ml, the method is well suited to quantify STX exposures based on previously reported cases. CONCLUSION: Compared with our previously published protocols, this method has improved selectivity, a fivefold increase in sensitivity and uses only a third of the sample volume. This method can diagnose future toxin exposures and may complement the shellfish monitoring programs worldwide.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saxitoxina / Pruebas Inmunológicas / Cromatografía Liquida / Espectrometría de Masas en Tándem / Intoxicación por Mariscos Tipo de estudio: Diagnostic_studies / Guideline Límite: Humans Idioma: En Revista: Bioanalysis Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saxitoxina / Pruebas Inmunológicas / Cromatografía Liquida / Espectrometría de Masas en Tándem / Intoxicación por Mariscos Tipo de estudio: Diagnostic_studies / Guideline Límite: Humans Idioma: En Revista: Bioanalysis Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido