Understanding protein-drug interactions using ion mobility-mass spectrometry.
Curr Opin Chem Biol
; 42: 167-176, 2018 02.
Article
en En
| MEDLINE
| ID: mdl-29331721
Ion mobility-mass spectrometry (IM-MS) is an important addition to the analytical toolbox for the structural evaluation of proteins, and is enhancing many areas of biophysical analysis. Disease-associated proteins, including enzymes such as protein kinases, transcription factors exemplified by p53, and intrinsically disordered proteins, including those prone to aggregation, are all amenable to structural analysis by IM-MS. In this review we discuss how this powerful technique can be used to understand protein conformational dynamics and aggregation pathways, and in particular, the effect that small molecules, including clinically-relevant drugs, play in these processes. We also present examples of how IM-MS can be used as a relatively rapid screening strategy to evaluate the mechanisms and conformation-driven aspects of protein:ligand interactions.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Quinasas
/
Espectrometría de Masas
/
Preparaciones Farmacéuticas
/
Proteínas
/
Proteínas Intrínsecamente Desordenadas
/
Espectrometría de Movilidad Iónica
Límite:
Humans
Idioma:
En
Revista:
Curr Opin Chem Biol
Asunto de la revista:
BIOQUIMICA
Año:
2018
Tipo del documento:
Article
Pais de publicación:
Reino Unido