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Transmembrane Pickets Connect Cyto- and Pericellular Skeletons Forming Barriers to Receptor Engagement.
Freeman, Spencer A; Vega, Anthony; Riedl, Magdalena; Collins, Richard F; Ostrowski, Phillip P; Woods, Elliot C; Bertozzi, Carolyn R; Tammi, Markku I; Lidke, Diane S; Johnson, Pauline; Mayor, Satyajit; Jaqaman, Khuloud; Grinstein, Sergio.
Afiliación
  • Freeman SA; Program in Cell Biology, Peter Gilgan Centre for Research and Learning, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
  • Vega A; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
  • Riedl M; Program in Cell Biology, Peter Gilgan Centre for Research and Learning, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
  • Collins RF; Program in Cell Biology, Peter Gilgan Centre for Research and Learning, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
  • Ostrowski PP; Program in Cell Biology, Peter Gilgan Centre for Research and Learning, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
  • Woods EC; Departments of Chemistry and Molecular Biology and Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Bertozzi CR; Departments of Chemistry and Molecular Biology and Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Tammi MI; Institute of Biomedicine, University of Eastern Finland, Kuopio 70210, Finland.
  • Lidke DS; Department of Pathology, Cancer Research Facility, School of Medicine, University of New Mexico, Albuquerque, NM 87131, USA.
  • Johnson P; Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
  • Mayor S; Cellular Organization and Signaling, National Centre for Biological Science, Tata Institute for Fundamental Research, Bangalore 560 065, India.
  • Jaqaman K; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
  • Grinstein S; Program in Cell Biology, Peter Gilgan Centre for Research and Learning, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Keenan Research Centre of the Li Ka Shing Knowledge Institute, St. Michael's Hospital, Toronto, ON M5C 1N8, Canada. Electronic address: sergio.grinstein@sickkids.ca.
Cell ; 172(1-2): 305-317.e10, 2018 01 11.
Article en En | MEDLINE | ID: mdl-29328918
Phagocytic receptors must diffuse laterally to become activated upon clustering by multivalent targets. Receptor diffusion, however, can be obstructed by transmembrane proteins ("pickets") that are immobilized by interacting with the cortical cytoskeleton. The molecular identity of these pickets and their role in phagocytosis have not been defined. We used single-molecule tracking to study the interaction between Fcγ receptors and CD44, an abundant transmembrane protein capable of indirect association with F-actin, hence likely to serve as a picket. CD44 tethers reversibly to formin-induced actin filaments, curtailing receptor diffusion. Such linear filaments predominate in the trailing end of polarized macrophages, where receptor mobility was minimal. Conversely, receptors were most mobile at the leading edge, where Arp2/3-driven actin branching predominates. CD44 binds hyaluronan, anchoring a pericellular coat that also limits receptor displacement and obstructs access to phagocytic targets. Force must be applied to traverse the pericellular barrier, enabling receptors to engage their targets.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citoesqueleto de Actina / Receptores Inmunológicos / Membrana Celular / Receptores de Hialuranos Límite: Adult / Animals / Female / Humans / Male Idioma: En Revista: Cell Año: 2018 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citoesqueleto de Actina / Receptores Inmunológicos / Membrana Celular / Receptores de Hialuranos Límite: Adult / Animals / Female / Humans / Male Idioma: En Revista: Cell Año: 2018 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos