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The protein kinase CK2 substrate Jabba modulates lipid metabolism during Drosophila oogenesis.
McMillan, Emily A; Longo, Sheila M; Smith, Michael D; Broskin, Sarah; Lin, Baicheng; Singh, Nisha K; Strochlic, Todd I.
Afiliación
  • McMillan EA; Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102.
  • Longo SM; Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102.
  • Smith MD; Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102.
  • Broskin S; Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102.
  • Lin B; Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102.
  • Singh NK; Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102.
  • Strochlic TI; Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102. Electronic address: Todd.Strochlic@drexelmed.edu.
J Biol Chem ; 293(8): 2990-3002, 2018 02 23.
Article en En | MEDLINE | ID: mdl-29326167
Lipid metabolism plays a critical role in female reproduction. During oogenesis, maturing oocytes accumulate high levels of neutral lipids that are essential for both energy production and the synthesis of other lipid molecules. Metabolic pathways within the ovary are partially regulated by protein kinases that link metabolic status to oocyte development. Although the functions of several kinases in this process are well established, the roles that many other kinases play in coordinating metabolic state with female germ cell development are unknown. Here, we demonstrate that the catalytic activity of casein kinase 2 (CK2) is essential for Drosophila oogenesis. Using an unbiased biochemical screen that leveraged an unusual catalytic property of the kinase, we identified a novel CK2 substrate in the Drosophila ovary, the lipid droplet-associated protein Jabba. We show that Jabba is essential for modulating ovarian lipid metabolism and for regulating female fertility in the fly. Our findings shed light on a CK2-dependent signaling pathway governing lipid metabolism in the ovary and provide insight into the long-recognized but poorly understood association between energy metabolism and female reproduction.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oogénesis / Ovario / Proteínas Portadoras / Regulación del Desarrollo de la Expresión Génica / Proteínas de Drosophila / Quinasa de la Caseína II / Metabolismo de los Lípidos Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: J Biol Chem Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oogénesis / Ovario / Proteínas Portadoras / Regulación del Desarrollo de la Expresión Génica / Proteínas de Drosophila / Quinasa de la Caseína II / Metabolismo de los Lípidos Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: J Biol Chem Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos