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Amoebal endosymbiont Neochlamydia protects host amoebae against Legionella pneumophila infection by preventing Legionella entry.
Maita, Chinatsu; Matsushita, Mizue; Miyoshi, Masahiro; Okubo, Torahiko; Nakamura, Shinji; Matsuo, Junji; Takemura, Masaharu; Miyake, Masaki; Nagai, Hiroki; Yamaguchi, Hiroyuki.
Afiliación
  • Maita C; Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, North-12, West-5, Kita-ku, Sapporo, 060-0812, Japan. Electronic address: chinatsu.d@gmail.com.
  • Matsushita M; Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, North-12, West-5, Kita-ku, Sapporo, 060-0812, Japan. Electronic address: all-around@eis.hokudai.ac.jp.
  • Miyoshi M; Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, North-12, West-5, Kita-ku, Sapporo, 060-0812, Japan. Electronic address: toruneko@eis.hokudai.ac.jp.
  • Okubo T; Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, North-12, West-5, Kita-ku, Sapporo, 060-0812, Japan. Electronic address: t.okubo@hs.hokudai.ac.jp.
  • Nakamura S; Division of Biomedical Imaging Research, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan. Electronic address: shinji-n@juntendo.ac.jp.
  • Matsuo J; Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, North-12, West-5, Kita-ku, Sapporo, 060-0812, Japan. Electronic address: matsuo@hs.hokudai.ac.jp.
  • Takemura M; Laboratory of Biology, Department of Liberal Arts, Faculty of Science, Tokyo University of Science (RIKADAI), Kagurazaka 1-3, Shinjuku, Tokyo, 162-8601, Japan. Electronic address: takemura@rs.kagu.tus.ac.jp.
  • Miyake M; Laboratory of Microbiology and Immunology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, 422-8526, Japan. Electronic address: miyakem@u-shizuoka-ken.ac.jp.
  • Nagai H; Gifu University School of Medicine, 1-1 Yanagido, Gifu City, 501-1193, Japan. Electronic address: hnagai@gifu-u.ac.jp.
  • Yamaguchi H; Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, North-12, West-5, Kita-ku, Sapporo, 060-0812, Japan. Electronic address: hiroyuki@med.hokudai.ac.jp.
Microbes Infect ; 20(4): 236-244, 2018 04.
Article en En | MEDLINE | ID: mdl-29317310
Acanthamoeba isolated from environmental soil harbors the obligate intracellular symbiont Neochlamydia, which has a critical role in host amoebal defense against Legionella pneumophila infection. Here, by using morphological analysis with confocal laser scanning fluorescence microscopy and transmission electron microscopy, proteome analyses with two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and liquid chromatography-mass spectrometry (LC/MS), and transcriptome analysis with DNA microarray, we explored the mechanism by which the Neochlamydia affected this defense. We observed that when rare uptake did occur, the symbiotic amoebae allowed Legionella to grow normally. However, the symbiotic amoebae had severely reduced uptake of Legionella when compared with the aposymbiotic amoebae. Also, in contrast to amoebae carrying the endosymbiont, the actin cytoskeleton was significantly disrupted by Legionella infection in aposymbiotic amoebae. Furthermore, despite Legionella exposure, there was little change in Neochlamydia gene expression. Taken together, we concluded that the endosymbiont, Neochlamydia prevents Legionella entry to the host amoeba, resulting in the host defense against Legionella infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Simbiosis / Legionella / Acanthamoeba / Chlamydiales Idioma: En Revista: Microbes Infect Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Francia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Simbiosis / Legionella / Acanthamoeba / Chlamydiales Idioma: En Revista: Microbes Infect Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Francia