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Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility.
de Souza, Wagner R; Martins, Polyana K; Freeman, Jackie; Pellny, Till K; Michaelson, Louise V; Sampaio, Bruno L; Vinecky, Felipe; Ribeiro, Ana P; da Cunha, Barbara A D B; Kobayashi, Adilson K; de Oliveira, Patricia A; Campanha, Raquel B; Pacheco, Thályta F; Martarello, Danielly C I; Marchiosi, Rogério; Ferrarese-Filho, Osvaldo; Dos Santos, Wanderley D; Tramontina, Robson; Squina, Fabio M; Centeno, Danilo C; Gaspar, Marília; Braga, Marcia R; Tiné, Marco A S; Ralph, John; Mitchell, Rowan A C; Molinari, Hugo B C.
Afiliación
  • de Souza WR; Embrapa Agroenergy, Brasília, DF, 70770901, Brazil.
  • Martins PK; Embrapa Agroenergy, Brasília, DF, 70770901, Brazil.
  • Freeman J; Plant Sciences, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK.
  • Pellny TK; Plant Sciences, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK.
  • Michaelson LV; Plant Sciences, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK.
  • Sampaio BL; Embrapa Agroenergy, Brasília, DF, 70770901, Brazil.
  • Vinecky F; Embrapa Agroenergy, Brasília, DF, 70770901, Brazil.
  • Ribeiro AP; Embrapa Agroenergy, Brasília, DF, 70770901, Brazil.
  • da Cunha BADB; Embrapa Agroenergy, Brasília, DF, 70770901, Brazil.
  • Kobayashi AK; Embrapa Agroenergy, Brasília, DF, 70770901, Brazil.
  • de Oliveira PA; Embrapa Agroenergy, Brasília, DF, 70770901, Brazil.
  • Campanha RB; Embrapa Agroenergy, Brasília, DF, 70770901, Brazil.
  • Pacheco TF; Embrapa Agroenergy, Brasília, DF, 70770901, Brazil.
  • Martarello DCI; Department of Biochemistry, State University of Maringá, Maringá, Paraná, 87020-900, Brazil.
  • Marchiosi R; Department of Biochemistry, State University of Maringá, Maringá, Paraná, 87020-900, Brazil.
  • Ferrarese-Filho O; Department of Biochemistry, State University of Maringá, Maringá, Paraná, 87020-900, Brazil.
  • Dos Santos WD; Department of Biochemistry, State University of Maringá, Maringá, Paraná, 87020-900, Brazil.
  • Tramontina R; Brazilian Bioethanol Science and Technology Laboratory, Brazilian Center for Research in Energy and Materials, Campinas, Sao Paulo, 13083-100, Brazil.
  • Squina FM; Programa de Processos Tecnológicos e Ambientais, Universidade de Sorocaba (UNISO), Sorocaba, 18060-000, Brazil.
  • Centeno DC; Centre of Natural Sciences and Humanities, Federal University of ABC, São Bernardo do Campo, SP, 09606-045, Brazil.
  • Gaspar M; Department of Plant Physiology and Biochemistry, Institute of Botany, Sao Paulo, 04301-012, 04301-902, Brazil.
  • Braga MR; Department of Plant Physiology and Biochemistry, Institute of Botany, Sao Paulo, 04301-012, 04301-902, Brazil.
  • Tiné MAS; Department of Plant Physiology and Biochemistry, Institute of Botany, Sao Paulo, 04301-012, 04301-902, Brazil.
  • Ralph J; Department of Biochemistry, University of Wisconsin, Madison, WI, 537, USA.
  • Mitchell RAC; Department of Energy's Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, WI, 537, USA.
  • Molinari HBC; Plant Sciences, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK.
New Phytol ; 218(1): 81-93, 2018 04.
Article en En | MEDLINE | ID: mdl-29315591
Feruloylation of arabinoxylan (AX) in grass cell walls is a key determinant of recalcitrance to enzyme attack, making it a target for improvement of grass crops, and of interest in grass evolution. Definitive evidence on the genes responsible is lacking so we studied a candidate gene that we identified within the BAHD acyl-CoA transferase family. We used RNA interference (RNAi) silencing of orthologs in the model grasses Setaria viridis (SvBAHD01) and Brachypodium distachyon (BdBAHD01) and determined effects on AX feruloylation. Silencing of SvBAHD01 in Setaria resulted in a c. 60% decrease in AX feruloylation in stems consistently across four generations. Silencing of BdBAHD01 in Brachypodium stems decreased feruloylation much less, possibly due to higher expression of functionally redundant genes. Setaria SvBAHD01 RNAi plants showed: no decrease in total lignin, approximately doubled arabinose acylated by p-coumarate, changes in two-dimensional NMR spectra of unfractionated cell walls consistent with biochemical estimates, no effect on total biomass production and an increase in biomass saccharification efficiency of 40-60%. We provide the first strong evidence for a key role of the BAHD01 gene in AX feruloylation and demonstrate that it is a promising target for improvement of grass crops for biofuel, biorefining and animal nutrition applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Supresión Genética / Coenzima A Transferasas / Pared Celular / Genes de Plantas / Biomasa / Ácidos Cumáricos / Setaria (Planta) Tipo de estudio: Etiology_studies Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Supresión Genética / Coenzima A Transferasas / Pared Celular / Genes de Plantas / Biomasa / Ácidos Cumáricos / Setaria (Planta) Tipo de estudio: Etiology_studies Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido