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Modulation of gingival cell response towards dental composites.
Jerg, A; Schulz, S D; Tomakidi, P; Hellwig, E; Polydorou, O.
Afiliación
  • Jerg A; Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetter Straße 55, 79106, Freiburg i. Br., Germany; Department of Oral Biotechnology, Center of Dental Medicine, Medical Center -
  • Schulz SD; Department of Oral Biotechnology, Center of Dental Medicine, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetter Straße 55, 79106, Freiburg i. Br., Germany.
  • Tomakidi P; Department of Oral Biotechnology, Center of Dental Medicine, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetter Straße 55, 79106, Freiburg i. Br., Germany.
  • Hellwig E; Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetter Straße 55, 79106, Freiburg i. Br., Germany.
  • Polydorou O; Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetter Straße 55, 79106, Freiburg i. Br., Germany. Electronic address: olga.polydorou@uniklinik-freiburg.de.
Dent Mater ; 34(3): 412-426, 2018 03.
Article en En | MEDLINE | ID: mdl-29306486
OBJECTIVE: This aim of this study was to investigate the cell behavioural response on clinically applied dental composites in exposition-relevant target cells, i.e. human gingival fibroblasts (HGF) and epithelial keratinocytes (HGK). METHODS: HGF and HGK were exposed to eluates of Ceram X™, Filtek™ Silorane, Filtek™ Supreme XTE, Fusio™ Liquid Dentin and Vertise™ Flow. Eluates were created by storing material samples in respective cell culture medium, for 24h and 72h (n=17), according to ISO 10993-12:2012. Cell response was evaluated at eluate exposure periods of 24h and 72h by (i) impedance analysis-based real-time monitoring of adhesion and proliferation, (ii) semi-quantitative indirect immunofluorescence (sq-IIF) detection of tissue-specific biomarkers, and (iii) ELISA-detection of pro-inflammatory interleukin (IL)-6. RESULTS: Generally, cell behavioural response towards the eluates was gradual in HGK and HGF, the latter exhibiting a less pronounced modulation per se. In HGK, ERK 1/2 was mainly activated after 24h by Fusio™ Liquid Dentin and Vertise™ Flow, while an increase in biomarker expression occurred time-delayed. A 72h exposure of HGK to eluates of Filtek™ Supreme XTE, Fusio™ Liquid Dentin and Vertise™ Flow significantly decreased secreted IL-6 amounts. In HGK, the impedance analysis revealed less proliferation and/or adhesion in case of Fusio™ Liquid Dentin and Vertise™ Flow with matched other composites. SIGNIFICANCE: In detail, protein expression and secretion is modulated particularly in terms of signal transduction, differentiation and inflammation. On cell biological level, all tested materials modulated the analysed features of cell behaviour with emphasis on the self-adhering composites.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Queratinocitos / Resinas Compuestas / Fibroblastos / Encía Límite: Humans Idioma: En Revista: Dent Mater Asunto de la revista: ODONTOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Queratinocitos / Resinas Compuestas / Fibroblastos / Encía Límite: Humans Idioma: En Revista: Dent Mater Asunto de la revista: ODONTOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido