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Transcoronary gradients of HDL-associated MicroRNAs in unstable coronary artery disease.
Choteau, Sébastien A; Cuesta Torres, Luisa F; Barraclough, Jennifer Y; Elder, Alexander M M; Martínez, Gonzalo J; Chen Fan, William Y; Shrestha, Sudichhya; Ong, Kwok L; Barter, Philip J; Celermajer, David S; Rye, Kerry-Anne; Patel, Sanjay; Tabet, Fatiha.
Afiliación
  • Choteau SA; School of Medical Sciences, University of New South Wales Australia, Wallace Wurth Building East, Randwick, Sydney, NSW 2031, Australia.
  • Cuesta Torres LF; School of Medical Sciences, University of New South Wales Australia, Wallace Wurth Building East, Randwick, Sydney, NSW 2031, Australia.
  • Barraclough JY; Department of Cardiology, Royal Prince Alfred Hospital, Missenden Road, Camperdown, Sydney, NSW 2050, Australia.
  • Elder AMM; Department of Cardiology, Royal Prince Alfred Hospital, Missenden Road, Camperdown, Sydney, NSW 2050, Australia.
  • Martínez GJ; División de Enfermedades Cardiovasculares, Pontificia Universidad Católica de Chile.
  • Chen Fan WY; School of Medical Sciences, University of New South Wales Australia, Wallace Wurth Building East, Randwick, Sydney, NSW 2031, Australia.
  • Shrestha S; School of Medical Sciences, University of New South Wales Australia, Wallace Wurth Building East, Randwick, Sydney, NSW 2031, Australia.
  • Ong KL; School of Medical Sciences, University of New South Wales Australia, Wallace Wurth Building East, Randwick, Sydney, NSW 2031, Australia.
  • Barter PJ; School of Medical Sciences, University of New South Wales Australia, Wallace Wurth Building East, Randwick, Sydney, NSW 2031, Australia.
  • Celermajer DS; Department of Cardiology, Royal Prince Alfred Hospital, Missenden Road, Camperdown, Sydney, NSW 2050, Australia.
  • Rye KA; School of Medical Sciences, University of New South Wales Australia, Wallace Wurth Building East, Randwick, Sydney, NSW 2031, Australia.
  • Patel S; Department of Cardiology, Royal Prince Alfred Hospital, Missenden Road, Camperdown, Sydney, NSW 2050, Australia.
  • Tabet F; School of Medical Sciences, University of New South Wales Australia, Wallace Wurth Building East, Randwick, Sydney, NSW 2031, Australia. Electronic address: f.tabet@unsw.edu.au.
Int J Cardiol ; 253: 138-144, 2018 02 15.
Article en En | MEDLINE | ID: mdl-29306454
AIMS: MicroRNAs (miRNAs) are transported on high-density lipoproteins (HDLs) and HDL-associated miRNAs are involved in intercellular communication. We explored HDL-associated miRNAs concentration gradients across the coronary circulation in stable and unstable coronary artery disease patients and whether changes in the transcoronary gradient were associated with changes in HDL composition and size. METHODS: Acute coronary syndrome (ACS, n=17) patients, those with stable coronary artery disease (stable CAD, n=19) and control subjects without CAD (n=6) were studied. HDLs were isolated from plasma obtained from the coronary sinus (CS), aortic root (arterial blood) and right atrium (venous blood). HDL-associated miRNAs (miR-16, miR-20a, miR-92a, miR-126, miR-222 and miR-223) were quantified by TaqMan miRNA assays. HDL particle sizes were determined by non-denaturing polyacrylamide gradient gel electrophoresis. HDL composition was measured immunoturbidometrically or enzymatically. RESULTS: A concentration gradient across the coronary circulation was observed for all the HDL-associated miRNAs. In ACS patients, there was a significant inverse transcoronary gradient for HDL-associated miR-16, miR-92a and miR-223 (p<0.05) compared to patients with stable CAD. Changes in HDL-miRNA transcoronary gradients were not associated with changes in HDL composition or size. CONCLUSION: HDLs are depleted of miR-16, miR-92a and miR-223 during the transcoronary passage in patients with ACS compared to patients with stable CAD.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de la Arteria Coronaria / MicroARNs / Síndrome Coronario Agudo / Oclusión Coronaria / Lipoproteínas HDL Tipo de estudio: Risk_factors_studies Límite: Female / Humans / Male Idioma: En Revista: Int J Cardiol Año: 2018 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de la Arteria Coronaria / MicroARNs / Síndrome Coronario Agudo / Oclusión Coronaria / Lipoproteínas HDL Tipo de estudio: Risk_factors_studies Límite: Female / Humans / Male Idioma: En Revista: Int J Cardiol Año: 2018 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos