Transmission versus reflectance spectroscopy for quantitation.
J Biomed Opt
; 23(1): 1-8, 2018 01.
Article
en En
| MEDLINE
| ID: mdl-29297210
The objective of this work was to compare the accuracy of analyte concentration estimation when using transmission versus diffuse reflectance spectroscopy of a scattering medium. Monte Carlo ray tracing of light through the medium was used in conjunction with pure component absorption spectra and Beer-Lambert absorption along each ray's pathlength to generate matched sets of pseudoabsorbance spectra, containing water and six analytes present in skin. PLS regression models revealed an improvement in accuracy when using transmission compared to reflectance for a range of medium thicknesses and instrument noise levels. An analytical expression revealed the source of the accuracy degradation with reflectance was due both to the reduced collection efficiency for a fixed instrument etendue and to the broad pathlength distribution that detected light travels in the medium before exiting from the incident side.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Piel
/
Espectroscopía Infrarroja Corta
Tipo de estudio:
Health_economic_evaluation
Límite:
Humans
Idioma:
En
Revista:
J Biomed Opt
Asunto de la revista:
ENGENHARIA BIOMEDICA
/
OFTALMOLOGIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos