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96 perfusable blood vessels to study vascular permeability in vitro.
van Duinen, V; van den Heuvel, A; Trietsch, S J; Lanz, H L; van Gils, J M; van Zonneveld, A J; Vulto, P; Hankemeier, T.
Afiliación
  • van Duinen V; Division of Analytical Biosciences, LACDR, Leiden University, Leiden, The Netherlands.
  • van den Heuvel A; Mimetas BV, Leiden, The Netherlands.
  • Trietsch SJ; Mimetas BV, Leiden, The Netherlands.
  • Lanz HL; Mimetas BV, Leiden, The Netherlands.
  • van Gils JM; Department of Internal Medicine, Einthoven laboratory for Vascular and Regenerative Medicine, LUMC, Leiden, The Netherlands.
  • van Zonneveld AJ; Department of Internal Medicine, Einthoven laboratory for Vascular and Regenerative Medicine, LUMC, Leiden, The Netherlands.
  • Vulto P; Mimetas BV, Leiden, The Netherlands.
  • Hankemeier T; Division of Analytical Biosciences, LACDR, Leiden University, Leiden, The Netherlands. hankemeier@lacdr.leidenuniv.nl.
Sci Rep ; 7(1): 18071, 2017 12 22.
Article en En | MEDLINE | ID: mdl-29273771
Current in vitro models to test the barrier function of vasculature are based on flat, two-dimensional monolayers. These monolayers do not have the tubular morphology of vasculature found in vivo and lack important environmental cues from the cellular microenvironment, such as interaction with an extracellular matrix (ECM) and exposure to flow. To increase the physiological relevance of in vitro models of the vasculature, it is crucial to implement these cues and better mimic the native three-dimensional vascular architecture. We established a robust, high-throughput method to culture endothelial cells as 96 three-dimensional and perfusable microvessels and developed a quantitative, real-time permeability assay to assess their barrier function. Culture conditions were optimized for microvessel formation in 7 days and were viable for over 60 days. The microvessels exhibited a permeability to 20 kDa dextran but not to 150 kDa dextran, which mimics the functionality of vasculature in vivo. Also, a dose-dependent effect of VEGF, TNFα and several cytokines confirmed a physiologically relevant response. The throughput and robustness of this method and assay will allow end-users in vascular biology to make the transition from two-dimensional to three-dimensional culture methods to study vasculature.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Permeabilidad Capilar / Endotelio Vascular / Microvasos / Células Endoteliales de la Vena Umbilical Humana Límite: Humans Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Permeabilidad Capilar / Endotelio Vascular / Microvasos / Células Endoteliales de la Vena Umbilical Humana Límite: Humans Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido