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Human Wharton's Jelly-Derived Stem Cells Display a Distinct Immunomodulatory and Proregenerative Transcriptional Signature Compared to Bone Marrow-Derived Stem Cells.
Donders, Raf; Bogie, Jeroen F J; Ravanidis, Stylianos; Gervois, Pascal; Vanheusden, Marjan; Marée, Raphaël; Schrynemackers, Marie; Smeets, Hubert J M; Pinxteren, Jef; Gijbels, Kristel; Walbers, Sara; Mays, Robert W; Deans, Robert; Van Den Bosch, Ludo; Stinissen, Piet; Lambrichts, Ivo; Gyselaers, Wilfried; Hellings, Niels.
Afiliación
  • Donders R; 1 Biomedical Research Institute, Hasselt University , Diepenbeek, Belgium .
  • Bogie JFJ; 1 Biomedical Research Institute, Hasselt University , Diepenbeek, Belgium .
  • Ravanidis S; 1 Biomedical Research Institute, Hasselt University , Diepenbeek, Belgium .
  • Gervois P; 1 Biomedical Research Institute, Hasselt University , Diepenbeek, Belgium .
  • Vanheusden M; 1 Biomedical Research Institute, Hasselt University , Diepenbeek, Belgium .
  • Marée R; 2 University of Liège , GIGA Bioinformatics Core Facility, Liège, Belgium .
  • Schrynemackers M; 2 University of Liège , GIGA Bioinformatics Core Facility, Liège, Belgium .
  • Smeets HJM; 3 Maastricht UMC+, Department of Genetics and Cell Biology, Research School GROW and CARIM , Maastricht, the Netherlands .
  • Pinxteren J; 4 ReGenesys BVBA , Bio-Incubator Leuven, Heverlee, Belgium .
  • Gijbels K; 4 ReGenesys BVBA , Bio-Incubator Leuven, Heverlee, Belgium .
  • Walbers S; 4 ReGenesys BVBA , Bio-Incubator Leuven, Heverlee, Belgium .
  • Mays RW; 5 Department of Regenerative Medicine, Athersys, Inc. , Cleveland, Ohio.
  • Deans R; 5 Department of Regenerative Medicine, Athersys, Inc. , Cleveland, Ohio.
  • Van Den Bosch L; 6 KU Leuven, Laboratory of Neurobiology, Experimental Neurology and VIB, Center for Brain & Disease , Leuven, Belgium .
  • Stinissen P; 1 Biomedical Research Institute, Hasselt University , Diepenbeek, Belgium .
  • Lambrichts I; 1 Biomedical Research Institute, Hasselt University , Diepenbeek, Belgium .
  • Gyselaers W; 1 Biomedical Research Institute, Hasselt University , Diepenbeek, Belgium .
  • Hellings N; 7 Ziekenhuis Oost-Limburg , Campus St. Jan, Genk, Belgium .
Stem Cells Dev ; 27(2): 65-84, 2018 01 15.
Article en En | MEDLINE | ID: mdl-29267140
Mesenchymal stromal cells (MSCs) are multipotent stem cells with immunosuppressive and trophic support functions. While MSCs from different sources frequently display a similar appearance in culture, they often show differences in their surface marker and gene expression profiles. Although bone marrow is considered the "gold standard" tissue to isolate classical MSCs (BM-MSC), MSC-like cells are currently also derived from more easily accessible extra-embryonic tissues such as the umbilical cord. In this study, we defined the best way to isolate MSCs from the Wharton's jelly of the human umbilical cord (WJ-MSC) and assessed the mesenchymal and immunological phenotype of BM-MSC and WJ-MSC. Moreover, the gene expression profile of established WJ-MSC cultures was compared to two different bone marrow-derived stem cell populations (BM-MSC and multipotent adult progenitor cells or MAPC®). We observed that explant culturing of Wharton's jelly matrix is superior to collagenase tissue digestion for obtaining mesenchymal-like cells, with explant isolated cells displaying increased expansion potential. While being phenotypically similar to adult MSCs, WJ-MSC show a different gene expression profile. Gene ontology analysis revealed that genes associated with cell adhesion, proliferation, and immune system functioning are enriched in WJ-MSC. In vivo transplantation confirms their immune modulatory effect on T cells, similar to BM-MSC and MAPC. Furthermore, WJ-MSC intrinsically overexpress genes involved in neurotrophic support and their secretome induces neuronal maturation of SH-SY5Y neuroblastoma cells to a greater extent than BM-MSC. This signature makes WJ-MSC an attractive candidate for cell-based therapy in neurodegenerative and immune-mediated central nervous system disorders such as multiple sclerosis, Parkinson's disease, or amyotrophic lateral sclerosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de la Médula Ósea / Regulación de la Expresión Génica / Línea Celular Tumoral / Proliferación Celular / Inmunomodulación / Ontología de Genes Límite: Humans Idioma: En Revista: Stem Cells Dev Asunto de la revista: HEMATOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de la Médula Ósea / Regulación de la Expresión Génica / Línea Celular Tumoral / Proliferación Celular / Inmunomodulación / Ontología de Genes Límite: Humans Idioma: En Revista: Stem Cells Dev Asunto de la revista: HEMATOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Estados Unidos