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Anti-angiogenesis effect of ß-D-mannuronic acid (M2000) as a novel NSAID with immunosuppressive properties under experimental model.
Rastegari-Pouyani, Mohsen; Mostafaie, Ali; Mansouri, Kamran; Mortazavi-Jahromi, Seyed Shahabeddin; Mohammadi-Motlagh, Hamid-Reza; Mirshafiey, Abbas.
Afiliación
  • Rastegari-Pouyani M; Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Mostafaie A; Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Mansouri K; Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
  • Mortazavi-Jahromi SS; Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
  • Mohammadi-Motlagh HR; Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Mirshafiey A; Department of Cellular and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran.
Clin Exp Pharmacol Physiol ; 45(4): 370-376, 2018 04.
Article en En | MEDLINE | ID: mdl-29266560
Angiogenesis is a process through which new capillaries are formed from pre-existing ones, which contributes significantly to the pathogenesis of numerous diseases, such as cancer and chronic inflammatory disorders. The ß-D-mannuronic acid (M2000) is a novel non-steroidal anti-inflammatory drug (NSAID) with immunosuppressive effects and is a matrix metalloproteinase (MMP) inhibitor. This research aimed to study the anti-angiogenesis effects of M2000 under in vitro and in vivo models. The cytotoxic and anti-proliferative effects of M2000 were examined using the trypan blue method and the MTT assay, respectively. The 3D collagen-cytodex model and the chick chorioallantoic membrane (CAM) assay were then used to evaluate the anti-angiogenesis property of M2000. Cytotoxicity assay revealed that M2000 (at concentrations of less than 100 µg/mL) had no cytotoxic effect on human umbilical vein endothelial cells (HUVECs). It was also illustrated that M2000 had little or no anti-proliferative effect on HUVECs. In addition, the anti-angiogenesis effects of M2000 were shown to be marginal in the in vitro model and both significant and dose-dependent in the in vivo status. This study showed that M2000 could be considered as an anti-angiogenic molecule which more likely exerts its activity mainly via indirect effects on endothelial cells and its anti-inflammatory effects may partly be attributable to its anti-angiogenic activity. Therefore, it could be recommended as a candidate for prevention and treatment of cancer, chronic inflammatory diseases, and other angiogenesis-related disorders.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antiinflamatorios no Esteroideos / Inhibidores de la Angiogénesis / Ácidos Hexurónicos / Inmunosupresores Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Clin Exp Pharmacol Physiol Año: 2018 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Australia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antiinflamatorios no Esteroideos / Inhibidores de la Angiogénesis / Ácidos Hexurónicos / Inmunosupresores Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Clin Exp Pharmacol Physiol Año: 2018 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Australia