Your browser doesn't support javascript.
loading
[Effect of Myeloma-Derived Exosomes on Surface Activating Receptors of NK Cells].
Xiong, Wen-Jie; Liu, Huan-Xun; Shi, Dun-Yun; Lou, Jin; Zhang, Qiong-Li.
Afiliación
  • Xiong WJ; Department of Hematology, Shenzhen Second People's Hospital, Shenzhen 518035, Guangdong Province, China. E-mail:szxiongwenjie@163.com.
  • Liu HX; Department of Hematology, Shenzhen Second People's Hospital, Shenzhen 518035, Guangdong Province, China.
  • Shi DY; Shenzhen Institute of Hematology, Shenzhen Second People's Hospital, Shenzhen 518035, Guangdong Province, China.
  • Lou J; Shenzhen Institute of Hematology, Shenzhen Second People's Hospital, Shenzhen 518035, Guangdong Province, China.
  • Zhang QL; Shenzhen Institute of Hematology, Shenzhen Second People's Hospital, Shenzhen 518035, Guangdong Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 25(6): 1713-1717, 2017 Dec.
Article en Zh | MEDLINE | ID: mdl-29262903
OBJECTIVE: To investigate the effect of myeloma-derived exosomes on surface activating receptors of NK cells, and to explore the mechanism of the function defect of NK cells. METHODS: The exosomes from the supernatant of multiple myeloma cell lines RPMI8226 and U266 were extracted by ultracentrifugation, and the size of them was identified under electron microscope; the human primary NK cells were extracted, and were co-cultured with the myeloma-derived exosomes (40 µg/ml), then the expression levels of surface activating receptors NKp46, NKp30 and NKG2D of NK cells at 0,1,4 and 24 hours were detected by flow cytometry. RESULTS: The exosomes showed small vesicular, sized 30-100 nm under electron microscope. The expression of surface activating receptors of NK cells declined at different degree after co-cultured with myeloma-derived exosomes. CONCLUSION: Myeloma-derived exosomes can inhibit the expression of surface activating receptors of NK cells.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de Superficie Celular / Exosomas / Receptores de Células Asesinas Naturales / Mieloma Múltiple Límite: Humans Idioma: Zh Revista: Zhongguo Shi Yan Xue Ye Xue Za Zhi Asunto de la revista: HEMATOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de Superficie Celular / Exosomas / Receptores de Células Asesinas Naturales / Mieloma Múltiple Límite: Humans Idioma: Zh Revista: Zhongguo Shi Yan Xue Ye Xue Za Zhi Asunto de la revista: HEMATOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: China