Selective regulation of type II interferon-inducible genes by NPM1/nucleophosmin.

Abe, Mayumi; Lin, Jianhuang; Nagata, Kyosuke; Okuwaki, Mitsuru

Abe, Mayumi; Lin, Jianhuang; Nagata, Kyosuke; Okuwaki, Mitsuru.

Afiliación

Abe M; Faculty of Medicine, University of Tsukuba, Japan.
Lin J; PhD Program of Human Biology, School of Integrative and Global Majors, University of Tsukuba, Japan.
Nagata K; Faculty of Medicine, University of Tsukuba, Japan.
Okuwaki M; PhD Program of Human Biology, School of Integrative and Global Majors, University of Tsukuba, Japan.

FEBS Lett ; 592(2): 244-255, 2018 01.

Article en En | MEDLINE | ID: mdl-29251779

RESUMEN

Nucleophosmin (NPM1) is a multifunctional nucleolar protein. Here, we analyze the role of NPM1 in gene expression using our previous microarray data and find a relationship between NPM1 and interferon (IFN)-Î³-inducible genes. We show that NPM1 selectively regulates the expression of a subset of IFN-Î³-inducible genes and directly binds to two important transcription factors in the type II IFN pathway: signal transducer and activator of transcription 1 and interferon regulatory factor 1 (IRF1). Furthermore, NPM1 is found to regulate the IFN-Î³-inducible promoter activity of major histocompatibility complex class II transactivator (CIITA), and mutation of the IRF1-binding site on the CIITA promoter abolishes the effect of NPM1. Our results suggest a novel mechanism for IFN-Î³-mediated gene expression by NPM1.

Asunto(s)

Perfilación de la Expresión Génica/métodos; Interferón gamma/farmacología; Proteínas Nucleares/metabolismo; Regulación de la Expresión Génica/efectos de los fármacos; Células HEK293; Células HeLa; Humanos; Factor 1 Regulador del Interferón/genética; Proteínas Nucleares/genética; Nucleofosmina; Regiones Promotoras Genéticas; Factor de Transcripción STAT1/genética; Transactivadores/genética

Palabras clave

JAK/STAT pathway; NPM1; STAT1; class II transactivator; interferon regulatory factor 1; interferon signaling pathway

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Nucleares / Interferón gamma / Perfilación de la Expresión Génica Límite: Humans Idioma: En Revista: FEBS Lett Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido

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