ppGpp Controls Global Gene Expression in Light and in Darkness in S. elongatus.

Puszynska, Anna M; O'Shea, Erin K

ppGpp Controls Global Gene Expression in Light and in Darkness in S. elongatus.

Puszynska, Anna M; O'Shea, Erin K.

Afiliación

Puszynska AM; Howard Hughes Medical Institute, Chevy Chase, MD, USA; Department of Molecular and Cell Biology, Harvard University, Cambridge, MA 02138, USA; Faculty of Arts and Sciences Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA.
O'Shea EK; Howard Hughes Medical Institute, Chevy Chase, MD, USA; Department of Molecular and Cell Biology, Harvard University, Cambridge, MA 02138, USA; Faculty of Arts and Sciences Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA. Electronic address: osheae@hhmi.org.

Cell Rep ; 21(11): 3155-3165, 2017 Dec 12.

Article en En | MEDLINE | ID: mdl-29241543

RESUMEN

The bacterial and plant stringent response involves production of the signaling molecules guanosine tetraphosphate and guanosine pentaphosphate ((p)ppGpp), leading to global reorganization of gene expression. The function of the stringent response has been well characterized in stress conditions, but its regulatory role during unstressed growth is less studied. Here, we demonstrate that (p)ppGpp-deficient strains of S. elongatus have globally deregulated biosynthetic capacity, with increased transcription rate, translation rate, and cell size in unstressed conditions in light and impaired viability in darkness. Synthetic restoration of basal guanosine tetraphosphate (ppGpp) levels is sufficient to recover transcriptional balance and appropriate cell size in light and to rescue viability in light/dark conditions, but it is insufficient to enable efficient dark-induced transcriptional shutdown. Our work underscores the importance of basal ppGpp signaling for regulation of cyanobacterial physiology in the absence of stress and for viability in energy-limiting conditions, highlighting that basal (p)ppGpp level is essential in cyanobacteria in the environmental light/dark cycle.

Asunto(s)

Proteínas Bacterianas/genética; Regulación Bacteriana de la Expresión Génica; Guanosina Pentafosfato/metabolismo; Ligasas/genética; Fototransducción/genética; Synechococcus/genética; Proteínas Bacterianas/metabolismo; Oscuridad; Ligasas/deficiencia; Viabilidad Microbiana/efectos de la radiación; Biosíntesis de Proteínas; Synechococcus/metabolismo; Synechococcus/efectos de la radiación; Transcripción Genética

Palabras clave

(p)ppGpp; PCC7942; Synechococcus elongatus; cyanobacteria; gene expression; light/dark cycle; stringent response

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Regulación Bacteriana de la Expresión Génica / Synechococcus / Fototransducción / Guanosina Pentafosfato / Ligasas Idioma: En Revista: Cell Rep Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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