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Use of autologous adipose-derived mesenchymal stem cells for creation of laryngeal cartilage.
Zhang, Hongji; Voytik-Harbin, Sherry; Brookes, Sarah; Zhang, Lujuan; Wallace, Joseph; Parker, Noah; Halum, Stacey.
Afiliación
  • Zhang H; Department of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, Indiana.
  • Voytik-Harbin S; Department of Biomedical Engineering, Purdue University, West Lafayette, Indiana.
  • Brookes S; Department of Biomedical Engineering, Purdue University, West Lafayette, Indiana.
  • Zhang L; Department of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, Indiana.
  • Wallace J; Department of Biomedical Engineering, Indiana University-Purdue University Indianapolis, Indianapolis, Indiana.
  • Parker N; Department of Speech and Hearing, Indiana University, Bloomington, Indiana, U.S.A.
  • Halum S; Department of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, Indiana.
Laryngoscope ; 128(4): E123-E129, 2018 04.
Article en En | MEDLINE | ID: mdl-29238978
OBJECTIVES/HYPOTHESIS: Adipose-derived mesenchymal stem cells (ASCs) are an exciting potential cell source for tissue engineering because cells can be derived from the simple excision of autologous fat. This study introduces a novel approach for tissue-engineering cartilage from ASCs and a customized collagen oligomer solution, and demonstrates that the resultant cartilage can be used for laryngeal cartilage reconstruction in an animal model. STUDY DESIGN: Basic science experimental design. METHODS: ASCs were isolated from F344 rats, seeded in a customized collagen matrix, and cultured in chondrogenic differentiation medium for 1, 2, and 4 weeks until demonstrating cartilage-like characteristics in vitro. Large laryngeal cartilage defects were created in the F344 rat model, with the engineered cartilage used to replace the cartilage defects, and the rats followed for 1 to 3 months. Staining examined cellular morphology and cartilage-specific features. RESULTS: In vitro histological staining revealed rounded chondrocyte-appearing cells evenly residing throughout the customized collagen scaffold, with positive staining for cartilage-specific markers. The cartilage was used to successfully repair large cartilaginous defects in the rat model, with excellent functional results. CONCLUSIONS: This study is the first study to demonstrate, in an animal model, that ASCs cultured in a unique form of collagen oligomer can create functional cartilage-like grafts that can be successfully used for partial laryngeal cartilage replacement. LEVEL OF EVIDENCE: NA. Laryngoscope, 128:E123-E129, 2018.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tejido Adiposo / Ingeniería de Tejidos / Trasplante de Células Madre Mesenquimatosas / Cartílagos Laríngeos / Laringectomía Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Laryngoscope Asunto de la revista: OTORRINOLARINGOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tejido Adiposo / Ingeniería de Tejidos / Trasplante de Células Madre Mesenquimatosas / Cartílagos Laríngeos / Laringectomía Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Laryngoscope Asunto de la revista: OTORRINOLARINGOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos