Your browser doesn't support javascript.
loading
Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function.
Zorzi, Veronica; Paciello, Fabiola; Ziraldo, Gaia; Peres, Chiara; Mazzarda, Flavia; Nardin, Chiara; Pasquini, Miriam; Chiani, Francesco; Raspa, Marcello; Scavizzi, Ferdinando; Carrer, Andrea; Crispino, Giulia; Ciubotaru, Catalin D; Monyer, Hannah; Fetoni, Anna R; M Salvatore, Anna; Mammano, Fabio.
Afiliación
  • Zorzi V; CNR Institute of Cell Biology and Neurobiology, Monterotondo, Italy.
  • Paciello F; School of Medicine, Institute of Otolaryngology, Catholic University, Rome, Italy.
  • Ziraldo G; CNR Institute of Cell Biology and Neurobiology, Monterotondo, Italy.
  • Peres C; CNR Institute of Cell Biology and Neurobiology, Monterotondo, Italy.
  • Mazzarda F; School of Medicine, Institute of Otolaryngology, Catholic University, Rome, Italy.
  • Nardin C; CNR Institute of Cell Biology and Neurobiology, Monterotondo, Italy.
  • Pasquini M; CNR Institute of Cell Biology and Neurobiology, Monterotondo, Italy.
  • Chiani F; Department of Science, Roma Tre University, Rome, Italy.
  • Raspa M; CNR Institute of Cell Biology and Neurobiology, Monterotondo, Italy.
  • Scavizzi F; Department of Science, Roma Tre University, Rome, Italy.
  • Carrer A; CNR Institute of Cell Biology and Neurobiology, Monterotondo, Italy.
  • Crispino G; Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Rome, Italy.
  • Ciubotaru CD; CNR Institute of Cell Biology and Neurobiology, Monterotondo, Italy.
  • Monyer H; CNR Institute of Cell Biology and Neurobiology, Monterotondo, Italy.
  • Fetoni AR; CNR Institute of Cell Biology and Neurobiology, Monterotondo, Italy.
  • M Salvatore A; Department of Physics and Astronomy G. Galilei, University of Padua, Padua, Italy.
  • Mammano F; Department of Physics and Astronomy G. Galilei, University of Padua, Padua, Italy.
Front Mol Neurosci ; 10: 379, 2017.
Article en En | MEDLINE | ID: mdl-29234270
Panx1 forms plasma membrane channels in brain and several other organs, including the inner ear. Biophysical properties, activation mechanisms and modulators of Panx1 channels have been characterized in detail, however the impact of Panx1 on auditory function is unclear due to conflicts in published results. To address this issue, hearing performance and cochlear function of the Panx1-/- mouse strain, the first with a reported global ablation of Panx1, were scrutinized. Male and female homozygous (Panx1-/-), hemizygous (Panx1+/-) and their wild type (WT) siblings (Panx1+/+) were used for this study. Successful ablation of Panx1 was confirmed by RT-PCR and Western immunoblotting in the cochlea and brain of Panx1-/- mice. Furthermore, a previously validated Panx1-selective antibody revealed strong immunoreactivity in WT but not in Panx1-/- cochleae. Hearing sensitivity, outer hair cell-based "cochlear amplifier" and cochlear nerve function, analyzed by auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) recordings, were normal in Panx1+/- and Panx1-/- mice. In addition, we determined that global deletion of Panx1 impacts neither on connexin expression, nor on gap-junction coupling in the developing organ of Corti. Finally, spontaneous intercellular Ca2+ signal (ICS) activity in organotypic cochlear cultures, which is key to postnatal development of the organ of Corti and essential for hearing acquisition, was not affected by Panx1 ablation. Therefore, our results provide strong evidence that, in mice, Panx1 is dispensable for hearing acquisition and auditory function.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Mol Neurosci Año: 2017 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Mol Neurosci Año: 2017 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza