A dominant-interfering camta3 mutation compromises primary transcriptional outputs mediated by both cell surface and intracellular immune receptors in Arabidopsis thaliana.

Jacob, Florence; Kracher, Barbara; Mine, Akira; Seyfferth, Carolin; Blanvillain-Baufumé, Servane; Parker, Jane E; Tsuda, Kenichi; Schulze-Lefert, Paul; Maekawa, Takaki

Jacob, Florence; Kracher, Barbara; Mine, Akira; Seyfferth, Carolin; Blanvillain-Baufumé, Servane; Parker, Jane E; Tsuda, Kenichi; Schulze-Lefert, Paul; Maekawa, Takaki.

Afiliación

Jacob F; Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829, Cologne, Germany.
Kracher B; Institute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Université Paris-Saclay, 91405, Orsay, France.
Mine A; Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829, Cologne, Germany.
Seyfferth C; Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829, Cologne, Germany.
Blanvillain-Baufumé S; Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829, Cologne, Germany.
Parker JE; Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829, Cologne, Germany.
Tsuda K; Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829, Cologne, Germany.
Schulze-Lefert P; Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829, Cologne, Germany.
Maekawa T; Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829, Cologne, Germany.

New Phytol ; 217(4): 1667-1680, 2018 03.

Article en En | MEDLINE | ID: mdl-29226970

RESUMEN

Pattern recognition receptors (PRRs) and nucleotide-binding domain and leucine-rich repeat (LRR)-containing proteins (NLRs) initiate pattern-triggered immunity (PTI) and effector-triggered immunity (ETI), respectively, each associated with the activation of an overlapping set of defence genes. The regulatory mechanism behind this convergence of PTI- and ETI-mediated defence gene induction remains elusive. We generated transgenic Arabidopsis plants that enable conditional NLR activation without pathogen infection to dissect NLR- and PRR-mediated transcriptional signals. A comparative analysis of over 40 transcriptome datasets linked calmodulin-binding transcription activators (CAMTAs) to the activation of overlapping defence genes in PTI and ETI. We used a dominant camta3 mutant (camta3-D) to assess CAMTA functions in the corresponding transcriptional regulation. Transcriptional regulation by NLRs, although highly similar to PTI responses, can be established independently of pathogen-associated molecular pattern (PAMP) perception, defence phytohormones and host cell death. Conditional expression of the N-terminal coiled-coil domain of the barley MLA (Mildew resistance locus A) NLR is sufficient to trigger similar transcriptional reprogramming as full-length NLRs. CAMTA-binding motifs are overrepresented in the 5' regulatory regions of the identified primary immune response genes, consistent with their altered expression and disease resistance responses in camta3-D plants. We propose that CAMTA-mediated transcriptional regulation defines an early convergence point in NLR- and PRR-mediated signalling.

Asunto(s)

Proteínas de Arabidopsis/genética; Arabidopsis/genética; Genes Dominantes; Espacio Intracelular/metabolismo; Mutación/genética; Receptores Inmunológicos/metabolismo; Factores de Transcripción/genética; Transcripción Genética; Arabidopsis/inmunología; Arabidopsis/microbiología; Proteínas de Arabidopsis/metabolismo; Resistencia a la Enfermedad/genética; Regulación de la Expresión Génica de las Plantas; Genes de Plantas; Proteínas NLR/metabolismo; Enfermedades de las Plantas/genética; Enfermedades de las Plantas/inmunología; Enfermedades de las Plantas/microbiología; Inmunidad de la Planta; Receptores de Reconocimiento de Patrones/metabolismo; Transducción de Señal; Estrés Fisiológico/genética; Factores de Transcripción/metabolismo; Transcriptoma/genética; Regulación hacia Arriba/genética

Palabras clave

Arabidopsis thaliana; calmodulin-binding transcription activator (CAMTA); cross-tolerance; effector-triggered immunity (ETI); immediate early genes; nucleotide-binding domain and LRR-containing proteins (NLRs); pattern-triggered immunity (PTI); transcriptional responses

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Transcripción Genética / Receptores Inmunológicos / Arabidopsis / Proteínas de Arabidopsis / Espacio Intracelular / Genes Dominantes / Mutación Tipo de estudio: Prognostic_studies Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2018 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido

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