Carnitine cycle in brown adipose tissue mitochondria as a tool for studying the regulatory role of fatty acids in the uncoupling protein function.
FEBS Lett
; 243(1): 37-40, 1989 Jan 16.
Article
en En
| MEDLINE
| ID: mdl-2920823
A concerted function of purine nucleotide (PN) binding and fatty acid (FA) release from the uncoupling protein (UP) resulting in the maximum coupling (potential) of brown adipose tissue (BAT) mitochondria was demonstrated. The uncoupling effect of FA was studied (at 4 mM MgCl2): 17 nmol oleate per mg protein caused a slight uncoupling with 8.9 mM ATP but with ATP below 3.6 mM almost total uncoupling was achieved. This shows that the PN-controlled gate can be stabilized in the closed conformation (with 8.9 mM ATP), also when FA is bound to UP. The sensitivity of the FA effect to ATP proves that oleate directly interacts with UP. The closed conformation of the H+ channel of UP is then abolished by oleate when a lower free ATP concentration is maintained outside.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Desacopladores
/
Tejido Adiposo Pardo
/
Ácidos Oléicos
/
Carnitina
/
Proteínas Portadoras
/
Ácidos Grasos no Esterificados
/
Proteínas de la Membrana
/
Mitocondrias
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1989
Tipo del documento:
Article
Pais de publicación:
Reino Unido