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The functional and molecular studies on involvement of hydrogen sulphide in myometrial activity of non-pregnant buffaloes (Bubalus bubalis).
Nair, Sooraj V; Sharma, Vipin; Sharma, Abhishek; Nakade, Udayraj P; Jaitley, Pooja; Mathesh, Karikalan; Choudhury, Soumen; Garg, Satish Kumar.
Afiliación
  • Nair SV; Experimental and Molecular Pharmacology Laboratory, Department of Pharmacology & Toxicology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura, 281001, India.
  • Sharma V; Experimental and Molecular Pharmacology Laboratory, Department of Pharmacology & Toxicology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura, 281001, India.
  • Sharma A; Experimental and Molecular Pharmacology Laboratory, Department of Pharmacology & Toxicology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura, 281001, India.
  • Nakade UP; Experimental and Molecular Pharmacology Laboratory, Department of Pharmacology & Toxicology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura, 281001, India.
  • Jaitley P; Experimental and Molecular Pharmacology Laboratory, Department of Pharmacology & Toxicology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura, 281001, India.
  • Mathesh K; Centre for Wildlife Conservation Management and Disease Surveillance, Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, Bareilly, U.P, 243 122, India.
  • Choudhury S; Experimental and Molecular Pharmacology Laboratory, Department of Pharmacology & Toxicology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura, 281001, India.
  • Garg SK; Experimental and Molecular Pharmacology Laboratory, Department of Pharmacology & Toxicology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura, 281001, India. profsatish@gmail.c
BMC Vet Res ; 13(1): 379, 2017 Dec 06.
Article en En | MEDLINE | ID: mdl-29207994
BACKGROUND: Hydrogen sulphide (H2S), a member of the gasotransmitters family, is known to play patho-physiological role in different body systems including during pregnancy. But its involvement in myometrial spontaneity and associated signalling pathways in uterus in non-pregnant animals is yet to be studied. Present study describes the effect of L-cysteine, an endogenous H2S donor, on isolated myometrial strips of non-pregnant buffaloes and the underlying signaling mechanism(s). RESULTS: L-cysteine (10 nM-30 mM) produced concentration-dependent contractile effect on buffalo myometrium which was extracellular Ca2+ and L-type calcium channels-dependent. Significant rightward shift of dose-response curve of L-cysteine was observed with significant decrease in maxima in the presence of amino-oxyacetic acid (AOAA; 100 µM) and d, l-propargylglycine (PAG; 100 µM), the specific blockers of cystathionine ß-synthase (CBS) and cystathionine γ-lyase (CSE), respectively. Existence of CBS enzyme of 63 kDa and CSE of 45 kDa molecular weights was confirmed by western blot using specific antibodies and also by immunohistochemistry. CONCLUSIONS: Endogenous H2S along with its biosynthetic enzymes (CBS and CSE) is evidently present in uteri of non-pregnant buffaloes and it regulates spontaneity in uteri of non-pregnant buffaloes and this effect is dependent on extracellular Ca2+ influx through nifedipine-sensitive L-type calcium channels. Thus H2S-signalling pathway may be a potential target to alter the uterine activities in physiology and patho-physiolgical states.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Búfalos / Sulfuro de Hidrógeno / Miometrio Límite: Animals Idioma: En Revista: BMC Vet Res Asunto de la revista: MEDICINA VETERINARIA Año: 2017 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Búfalos / Sulfuro de Hidrógeno / Miometrio Límite: Animals Idioma: En Revista: BMC Vet Res Asunto de la revista: MEDICINA VETERINARIA Año: 2017 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido