Dark-chilling and subsequent photo-activation modulate expression and induce reversible association of chloroplast lipoxygenase with thylakoid membrane in runner bean (Phaseolus coccineus L.).

Mazur, Radoslaw; Trzcinska-Danielewicz, Joanna; Kozlowski, Piotr; Kowalewska, Lucja; Rumak, Izabela; Shiell, Brian J; Mostowska, Agnieszka; Michalski, Wojtek P; Garstka, Maciej

Mazur, Radoslaw; Trzcinska-Danielewicz, Joanna; Kozlowski, Piotr; Kowalewska, Lucja; Rumak, Izabela; Shiell, Brian J; Mostowska, Agnieszka; Michalski, Wojtek P; Garstka, Maciej.

Afiliación

Mazur R; Department of Metabolic Regulation, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland. Electronic address: rmazur@biol.uw.edu.pl.
Trzcinska-Danielewicz J; Department of Molecular Biology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Kozlowski P; Department of Molecular Biology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Kowalewska L; Department of Plant Anatomy and Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Rumak I; Department of Plant Anatomy and Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Shiell BJ; CSIRO Australian Animal Health Laboratory, Private Bag 24, Geelong, VIC 3220, Australia.
Mostowska A; Department of Plant Anatomy and Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Michalski WP; CSIRO Australian Animal Health Laboratory, Private Bag 24, Geelong, VIC 3220, Australia.
Garstka M; Department of Metabolic Regulation, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.

Plant Physiol Biochem ; 122: 102-112, 2018 Jan.

Article en En | MEDLINE | ID: mdl-29207281

RESUMEN

Lipoxygenases (LOXs) are non-haem iron-containing dioxygenases that catalyse oxygenation of polyunsaturated fatty acids. This reaction is the first step in biosynthesis of oxylipins, which play important and diverse roles in stress response. In this study, we identified four LOX genes (PcLOXA, B, C, D) in chilling-sensitive runner bean (Phaseolus coccineus L.) plant and analyzed their expression patterns during long term dark-chilling (4 °C) stress and during day/night (21ºC/4 °C) temperature fluctuations. Three of the four identified LOX genes, namely PcLOXA, PcLOXB and PcLOXD, were induced by wounding stress, while only the PcLOXA was induced by dark-chilling of both detached (wounded) leaves and whole plants. We identified PcLOXA as a chloroplast-targeted LOX protein and investigated its expression during chilling stress in terms of abundance, localization inside chloroplasts and interactions with the thylakoid membranes. The analysis by immunogold electron microscopy has shown that more than 60% of detectable PcLOXA protein was associated with thylakoids, and dark-chilling of leaves resulted in increased amounts of this protein detected within grana margins of thylakoids. This effect was reversible under subsequent photo-activation of chilled leaves. PcLOXA binding to thylakoids is not mediated by the posttranslational modification but rather is based on direct interactions of the protein with membrane lipids; the binding strength increases under dark-chilling conditions.

Asunto(s)

Frío; Luz; Lipooxigenasa/metabolismo; Phaseolus/enzimología; Proteínas de Plantas/metabolismo; Tilacoides/enzimología

Palabras clave

Chilling stress; Gene and protein expression; Lipoxygenase; Phaseolus coccineus; Thylakoid membrane

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Lipooxigenasa / Frío / Tilacoides / Phaseolus / Luz Tipo de estudio: Risk_factors_studies Idioma: En Revista: Plant Physiol Biochem Asunto de la revista: BIOQUIMICA / BOTANICA Año: 2018 Tipo del documento: Article Pais de publicación: Francia

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