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Local Signals in Mouse Horizontal Cell Dendrites.
Chapot, Camille A; Behrens, Christian; Rogerson, Luke E; Baden, Tom; Pop, Sinziana; Berens, Philipp; Euler, Thomas; Schubert, Timm.
Afiliación
  • Chapot CA; Institute for Ophthalmic Research, University of Tübingen, 72076 Tübingen, Germany; Center for Integrative Neuroscience, University of Tübingen, 72076 Tübingen, Germany; Graduate Training Centre of Neuroscience, University of Tübingen, 72076 Tübingen, Germany.
  • Behrens C; Institute for Ophthalmic Research, University of Tübingen, 72076 Tübingen, Germany; Center for Integrative Neuroscience, University of Tübingen, 72076 Tübingen, Germany; Graduate Training Centre of Neuroscience, University of Tübingen, 72076 Tübingen, Germany; Bernstein Center for Computational Neur
  • Rogerson LE; Institute for Ophthalmic Research, University of Tübingen, 72076 Tübingen, Germany; Center for Integrative Neuroscience, University of Tübingen, 72076 Tübingen, Germany; Graduate Training Centre of Neuroscience, University of Tübingen, 72076 Tübingen, Germany; Bernstein Center for Computational Neur
  • Baden T; Institute for Ophthalmic Research, University of Tübingen, 72076 Tübingen, Germany; School of Life Sciences, University of Sussex, Brighton BN1 9RH, UK.
  • Pop S; Institute for Ophthalmic Research, University of Tübingen, 72076 Tübingen, Germany; Center for Integrative Neuroscience, University of Tübingen, 72076 Tübingen, Germany; Graduate Training Centre of Neuroscience, University of Tübingen, 72076 Tübingen, Germany.
  • Berens P; Institute for Ophthalmic Research, University of Tübingen, 72076 Tübingen, Germany; Center for Integrative Neuroscience, University of Tübingen, 72076 Tübingen, Germany; Bernstein Center for Computational Neuroscience, University of Tübingen, 72076 Tübingen, Germany.
  • Euler T; Institute for Ophthalmic Research, University of Tübingen, 72076 Tübingen, Germany; Center for Integrative Neuroscience, University of Tübingen, 72076 Tübingen, Germany; Bernstein Center for Computational Neuroscience, University of Tübingen, 72076 Tübingen, Germany. Electronic address: thomas.euler
  • Schubert T; Institute for Ophthalmic Research, University of Tübingen, 72076 Tübingen, Germany; Center for Integrative Neuroscience, University of Tübingen, 72076 Tübingen, Germany. Electronic address: timm.schubert@cin.uni-tuebingen.de.
Curr Biol ; 27(23): 3603-3615.e5, 2017 Dec 04.
Article en En | MEDLINE | ID: mdl-29174891
The mouse retina contains a single type of horizontal cell, a GABAergic interneuron that samples from all cone photoreceptors within reach and modulates their glutamatergic output via parallel feedback mechanisms. Because horizontal cells form an electrically coupled network, they have been implicated in global signal processing, such as large-scale contrast enhancement. Recently, it has been proposed that horizontal cells can also act locally at the level of individual cone photoreceptors. To test this possibility physiologically, we used two-photon microscopy to record light stimulus-evoked Ca2+ signals in cone axon terminals and horizontal cell dendrites as well as glutamate release in the outer plexiform layer. By selectively stimulating the two mouse cone opsins with green and UV light, we assessed whether signals from individual cones remain isolated within horizontal cell dendritic tips or whether they spread across the dendritic arbor. Consistent with the mouse's opsin expression gradient, we found that the Ca2+ signals recorded from dendrites of dorsal horizontal cells were dominated by M-opsin and those of ventral horizontal cells by S-opsin activation. The signals measured in neighboring horizontal cell dendritic tips varied markedly in their chromatic preference, arguing against global processing. Rather, our experimental data and results from biophysically realistic modeling support the idea that horizontal cells can process cone input locally, extending the classical view of horizontal cell function. Pharmacologically removing horizontal cells from the circuitry reduced the sensitivity of the cone signal to low frequencies, suggesting that local horizontal cell feedback shapes the temporal properties of cone output.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Axones / Transducción de Señal / Células Fotorreceptoras Retinianas Conos / Dendritas / Ratones Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Curr Biol Asunto de la revista: BIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Axones / Transducción de Señal / Células Fotorreceptoras Retinianas Conos / Dendritas / Ratones Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Curr Biol Asunto de la revista: BIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido