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Migrating Myeloid Cells Sense Temporal Dynamics of Chemoattractant Concentrations.
Petrie Aronin, Caren E; Zhao, Yun M; Yoon, Justine S; Morgan, Nicole Y; Prüstel, Thorsten; Germain, Ronald N; Meier-Schellersheim, Martin.
Afiliación
  • Petrie Aronin CE; Laboratory of Systems Biology (LSB), Lymphocyte Biology Section (LBS), National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD 20892, USA.
  • Zhao YM; Laboratory of Systems Biology (LSB), Lymphocyte Biology Section (LBS), National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD 20892, USA.
  • Yoon JS; Biomedical Engineering and Physical Sciences Resource (BEPS), Microfabrication and Microfluidics Unit (MMU), National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA.
  • Morgan NY; Biomedical Engineering and Physical Sciences Resource (BEPS), Microfabrication and Microfluidics Unit (MMU), National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA.
  • Prüstel T; Laboratory of Systems Biology (LSB), Computational Biology Unit (CBU), National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD 20892, USA.
  • Germain RN; Laboratory of Systems Biology (LSB), Lymphocyte Biology Section (LBS), National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: rgermain@niaid.nih.gov.
  • Meier-Schellersheim M; Laboratory of Systems Biology (LSB), Computational Biology Unit (CBU), National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: mms@niaid.nih.gov.
Immunity ; 47(5): 862-874.e3, 2017 11 21.
Article en En | MEDLINE | ID: mdl-29166587
Chemoattractant-mediated recruitment of hematopoietic cells to sites of pathogen growth or tissue damage is critical to host defense and organ homeostasis. Chemotaxis is typically considered to rely on spatial sensing, with cells following concentration gradients as long as these are present. Utilizing a microfluidic approach, we found that stable gradients of intermediate chemokines (CCL19 and CXCL12) failed to promote persistent directional migration of dendritic cells or neutrophils. Instead, rising chemokine concentrations were needed, implying that temporal sensing mechanisms controlled prolonged responses to these ligands. This behavior was found to depend on G-coupled receptor kinase-mediated negative regulation of receptor signaling and contrasted with responses to an end agonist chemoattractant (C5a), for which a stable gradient led to persistent migration. These findings identify temporal sensing as a key requirement for long-range myeloid cell migration to intermediate chemokines and provide insights into the mechanisms controlling immune cell motility in complex tissue environments.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Movimiento Celular / Factores Quimiotácticos / Células Mieloides Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Immunity Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Movimiento Celular / Factores Quimiotácticos / Células Mieloides Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Immunity Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos