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Cell culture models of fatty acid overload: Problems and solutions.
Alsabeeh, Nour; Chausse, Bruno; Kakimoto, Pamela A; Kowaltowski, Alicia J; Shirihai, Orian.
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  • Alsabeeh N; Department of Medicine, Division of Endocrinology, Diabetes and Hypertension, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA; Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA 02118, USA; Department of Physiology, Faculty of Medicine, Kuwait
  • Chausse B; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil.
  • Kakimoto PA; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil.
  • Kowaltowski AJ; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil. Electronic address: alicia@iq.usp.br.
  • Shirihai O; Department of Medicine, Division of Endocrinology, Diabetes and Hypertension, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA.
Biochim Biophys Acta Mol Cell Biol Lipids ; 1863(2): 143-151, 2018 Feb.
Article en En | MEDLINE | ID: mdl-29155055
High plasma levels of fatty acids occur in a variety of metabolic diseases. Cellular effects of fatty acid overload resulting in negative cellular responses (lipotoxicity) are often studied in vitro, in an attempt to understand mechanisms involved in these diseases. Fatty acids are poorly soluble, and thus usually studied when complexed to albumins such as bovine serum albumin (BSA). The conjugation of fatty acids to albumin requires care pertaining to preparation of the solutions, effective free fatty acid concentrations, use of different fatty acid species, types of BSA, appropriate controls and ensuring cellular fatty acid uptake. This review discusses lipotoxicity models, the potential problems encountered when using these cellular models, as well as practical solutions for difficulties encountered.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Trastornos del Metabolismo de los Lípidos / Ácidos Grasos / Modelos Biológicos Límite: Animals / Humans Idioma: En Revista: Biochim Biophys Acta Mol Cell Biol Lipids Año: 2018 Tipo del documento: Article País de afiliación: Kuwait Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Trastornos del Metabolismo de los Lípidos / Ácidos Grasos / Modelos Biológicos Límite: Animals / Humans Idioma: En Revista: Biochim Biophys Acta Mol Cell Biol Lipids Año: 2018 Tipo del documento: Article País de afiliación: Kuwait Pais de publicación: Países Bajos