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O6-methylguanine-DNA-methyltransferase immunostaining intensity in glioblastoma.
Jiménez, Daniel; Matamala, José Manuel; Chiti, Alessandra; Vergara, Carmen; Tissera, Claudia; Melo, Romulo; Cartier, Luis.
Afiliación
  • Jiménez D; Laboratory of Biomedical Sciences, Department of Neurological Sciences, Faculty of Medicine, University of Chile, Santiago, Chile; Neurology Service, Hospital del Salvador, Santiago, Chile. Electronic address: danieljimenez@med.uchile.cl.
  • Matamala JM; Laboratory of Biomedical Sciences, Department of Neurological Sciences, Faculty of Medicine, University of Chile, Santiago, Chile; Biomedical Neuroscience Institute (BNI), Faculty of Medicine, University of Chile, Santiago, Chile.
  • Chiti A; Laboratory of Biomedical Sciences, Department of Neurological Sciences, Faculty of Medicine, University of Chile, Santiago, Chile.
  • Vergara C; Laboratory of Biomedical Sciences, Department of Neurological Sciences, Faculty of Medicine, University of Chile, Santiago, Chile.
  • Tissera C; Instituto de Neurocirugía Dr. Asenjo, Santiago, Chile.
  • Melo R; Instituto de Neurocirugía Dr. Asenjo, Santiago, Chile; Department of Neurological Science, Faculty of Medicine, University of Chile, Santiago, Chile.
  • Cartier L; Laboratory of Biomedical Sciences, Department of Neurological Sciences, Faculty of Medicine, University of Chile, Santiago, Chile; Neurology Service, Hospital del Salvador, Santiago, Chile.
Neurol Neurochir Pol ; 52(1): 116-119, 2018.
Article en En | MEDLINE | ID: mdl-29153917
Immunohistochemistry (IHC) for O6-methylguanine-DNA-methyltransferase (MGMT) has shown heterogeneous results. Cell staining intensity has not been included as a quantifiable variable in IHC analyses. We performed MGMT IHC in 29 patients diagnosed as glioblastoma classifying cells into three categories based on nuclear staining intensity compared with adjacent endothelium. The median proportions of strong-moderate, weak and no staining cells were 10%, 16% and 71%, respectively. The proportion of positive cases for MGMT expression varies from 38% to 52% depending on the classification of weakly stained cells. This letter challenges previous studies that have not included intensity as a variable for IHC analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Encefálicas / Metilasas de Modificación del ADN / Glioblastoma / Proteínas Supresoras de Tumor / Enzimas Reparadoras del ADN Límite: Humans Idioma: En Revista: Neurol Neurochir Pol Año: 2018 Tipo del documento: Article Pais de publicación: Polonia
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Encefálicas / Metilasas de Modificación del ADN / Glioblastoma / Proteínas Supresoras de Tumor / Enzimas Reparadoras del ADN Límite: Humans Idioma: En Revista: Neurol Neurochir Pol Año: 2018 Tipo del documento: Article Pais de publicación: Polonia