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Intraurethral injection with LPS: an effective experimental model of prostatic inflammation.
Dos Santos Gomes, Fabiana Oliveira; Oliveira, Amanda Costa; Ribeiro, Edlene Lima; da Silva, Bruna Santos; Dos Santos, Laise Aline Martins; de Lima, Ingrid Tavares; Silva, Amanda Karolina Soares E; da Rocha Araújo, Shyrlene Meiry; Gonçalves, Terezinha; de Melo-Junior, Mario Ribeiro; Peixoto, Christina Alves.
Afiliación
  • Dos Santos Gomes FO; Ultrastructure Laboratory, Instituto Aggeu Magalhães, Centro de Pesquisas Aggeu Magalhães (CPqAM-FIOCRUZ), Fundação Oswaldo Cruz, Av. Moraes Rego s/n, Cidade Universitária, Recife, PE, 50670-420, Brazil.
  • Oliveira AC; Pós-graduação em Ciências Biológicas da Universidade Federal de Pernambuco (UFPE), Recife, Brazil.
  • Ribeiro EL; Faculdade Integrada de Pernambuco (FACIPE), Recife, Brazil.
  • da Silva BS; Ultrastructure Laboratory, Instituto Aggeu Magalhães, Centro de Pesquisas Aggeu Magalhães (CPqAM-FIOCRUZ), Fundação Oswaldo Cruz, Av. Moraes Rego s/n, Cidade Universitária, Recife, PE, 50670-420, Brazil.
  • Dos Santos LAM; Pós-graduação em Ciências Biológicas da Universidade Federal de Pernambuco (UFPE), Recife, Brazil.
  • de Lima IT; Ultrastructure Laboratory, Instituto Aggeu Magalhães, Centro de Pesquisas Aggeu Magalhães (CPqAM-FIOCRUZ), Fundação Oswaldo Cruz, Av. Moraes Rego s/n, Cidade Universitária, Recife, PE, 50670-420, Brazil.
  • Silva AKSE; Pós-graduação em Ciências Biológicas da Universidade Federal de Pernambuco (UFPE), Recife, Brazil.
  • da Rocha Araújo SM; Faculdade Integrada de Pernambuco (FACIPE), Recife, Brazil.
  • Gonçalves T; Ultrastructure Laboratory, Instituto Aggeu Magalhães, Centro de Pesquisas Aggeu Magalhães (CPqAM-FIOCRUZ), Fundação Oswaldo Cruz, Av. Moraes Rego s/n, Cidade Universitária, Recife, PE, 50670-420, Brazil.
  • de Melo-Junior MR; Ultrastructure Laboratory, Instituto Aggeu Magalhães, Centro de Pesquisas Aggeu Magalhães (CPqAM-FIOCRUZ), Fundação Oswaldo Cruz, Av. Moraes Rego s/n, Cidade Universitária, Recife, PE, 50670-420, Brazil.
  • Peixoto CA; Pós-graduação em Ciências Biológicas da Universidade Federal de Pernambuco (UFPE), Recife, Brazil.
Inflamm Res ; 67(1): 43-55, 2018 Jan.
Article en En | MEDLINE | ID: mdl-29151155
OBJECTIVE: Chronic inflammation has been recognized as having a prominent role pathogenesis of benign prostatic hyperplasia (BPH) and cancer. It is believed that chronic inflammation induces prostatic fibromuscular growth. This correlation has been clearly illustrated by both in vivo and in vitro studies; however, current experimental models of BPH require complex surgery or hormonal treatment. Therefore, the aim of the present study was to propose a new murine model of BPH/prostatitis induced by intraurethral injection of LPS. METHODS: Male Swiss and C57Bl/6 mice were then sacrificed 3, 7, 10, and 14 days after intraurethral injection of LPS. The prostates were quickly dissected and fixed for morphological and immunohistochemical analyses. RESULTS: The results showed that LPS played an important role in the cell proliferation of the prostate. Histological and ultrastructural analysis showed epithelial hyperplasia, clear stromal cells, little inflammatory infiltration, and heavy bleeding. Treatment with LPS also promoted the increase of growth factor (FGF-7 and TGF-ß), α-actin, and proinflammatory cytokines (IL-1, IL-6, IL-17), both in the stroma and epithelium. CONCLUSION: According to the present findings, it can be concluded that the intraurethral administration of LPS promotes tissue remodeling, as well as stimulating the pattern of pro-inflammatory cytokines, and therefore, constitutes an effective experimental model of BPH/inflammation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Próstata / Hiperplasia Prostática / Lipopolisacáridos / Inflamación Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Inflamm Res Asunto de la revista: ALERGIA E IMUNOLOGIA / PATOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Próstata / Hiperplasia Prostática / Lipopolisacáridos / Inflamación Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Inflamm Res Asunto de la revista: ALERGIA E IMUNOLOGIA / PATOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza