Your browser doesn't support javascript.
loading
Induction of innervation by encapsulated adipocytes with engineered vitamin A metabolism.
Shen, Qiwen; Yasmeen, Rumana; Marbourg, Jessica; Xu, Lu; Yu, Lianbo; Fadda, Paolo; Flechtner, Alan; Lee, L James; Popovich, Phillip G; Ziouzenkova, Ouliana.
Afiliación
  • Shen Q; Department of Human Sciences, The Ohio State University, Columbus, Ohio.
  • Yasmeen R; Department of Human Sciences, The Ohio State University, Columbus, Ohio.
  • Marbourg J; Center for Brain and Spinal Cord Repair, The Ohio State University, Columbus, Ohio; Department of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, Ohio.
  • Xu L; Department of Human Sciences, The Ohio State University, Columbus, Ohio; Department of Minimally Invasive Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
  • Yu L; Department of Statistics, The Ohio State University College of Medicine, Columbus, Ohio.
  • Fadda P; Nucleic Acid Shared Resource, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio.
  • Flechtner A; Histology and Immunohistochemistry Laboratory, The Ohio State University, Columbus, Ohio.
  • Lee LJ; Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio.
  • Popovich PG; Center for Brain and Spinal Cord Repair, The Ohio State University, Columbus, Ohio; Department of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, Ohio.
  • Ziouzenkova O; Department of Human Sciences, The Ohio State University, Columbus, Ohio. Electronic address: ziouzenkova.1@osu.edu.
Transl Res ; 192: 1-14, 2018 02.
Article en En | MEDLINE | ID: mdl-29144959
Innervation is a fundamental basis for function and survival of tissues. In the peripheral tissues, degenerative diseases create a neurotoxic metabolic milieu that either causes neurodegeneration or fails to sustain regenerative growth and reinnervation of injured/diseased tissues. Encapsulation of cells producing neurotrophic factors can augment axon growth and neuron survival; however, sustained innervation in vivo requires a combination of factors promoting axon growth and guidance pathway that are released in a tissue-specific context. Using novel encapsulation techniques and genetic tools, we manipulated retinoic acid-generating enzyme aldehyde dehydrogenase 1a1 (Aldh1a1) in adipocytes that are capable of promoting growth and innervation of white adipose tissue by sympathetic neurons. Aldh1a1-/- adipocytes secrete molecules that regulate axon guidance and markedly stimulate neurite outgrowth in vitro and in vivo. Based on studies with natural and synthetic RAR agonists and antagonists, gene microarray and nanostring arrays, we concluded that ephrin A5/ephrin A4 is a downstream pathway regulated by Aldh1a1. Encapsulation of Aldh1a1-/- adipocytes into alginate poly-L-lysine microcapsules induced functional innervation of adipose tissue in obese wild-type mice. We propose that encapsulated Aldh1a1-/- adipocytes could provide a therapeutic solution for the reinnervation of damaged tissues.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistema Nervioso Simpático / Vitamina A / Adipocitos / Aldehído Deshidrogenasa / Tejido Adiposo Blanco Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: Transl Res Asunto de la revista: MEDICINA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistema Nervioso Simpático / Vitamina A / Adipocitos / Aldehído Deshidrogenasa / Tejido Adiposo Blanco Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: Transl Res Asunto de la revista: MEDICINA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos