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Comparative In Vitro Study of 11C-Methionine and 11C-Deuterodeprenyl Uptake in Three Human Glioma Cell Lines.
Vasilskis, Elena; Kreimerman, Ingrid; Olivera, Silvia; Savio, Eduardo; Engler, Henry.
Afiliación
  • Vasilskis E; 1 Biomedical Research Department, Centro Uruguayo de Imagenologia Molecular , Montevideo, Uruguay .
  • Kreimerman I; 2 Department of Radiopharmacy, Centro Uruguayo de Imagenologia Molecular , Montevideo, Uruguay .
  • Olivera S; 3 Instituto de Investigaciones Biologicas Clemente Estable , Montevideo, Uruguay .
  • Savio E; 1 Biomedical Research Department, Centro Uruguayo de Imagenologia Molecular , Montevideo, Uruguay .
  • Engler H; 2 Department of Radiopharmacy, Centro Uruguayo de Imagenologia Molecular , Montevideo, Uruguay .
Cancer Biother Radiopharm ; 32(9): 344-350, 2017 Nov.
Article en En | MEDLINE | ID: mdl-29140741
AIM: To compare the uptake of 11C-deuterodeprenyl (11C-DED) and 11C-methionine (11C-MET) in three human glioma cell lines and study the relationship with glial fibrillary acid protein (GFAP) and monoamine oxidase B (MAO B) expression. 11C-DED is used in positron emission tomography imaging as a marker of astrocytosis in various central nervous system pathologies. It binds irreversibly to MAO B, a glial dimeric enzyme with increased activity in some neurological pathologies. MATERIALS AND METHODS: Binding and internalization studies of 11C-MET and 11C-DED were performed in astrocytoma grade III, glioblastoma grade IV, and radio-resistant glioblastoma grade IV cells. Immunofluorescence was used. RESULTS: 11C-MET specific activity bound to membrane was 9.0%-11.1% and that internalized was 88.9%-91.0%. 11C-DED specific activity bound to membrane was 34.8%-58.0% and that internalized was 38.7%-65.2%. Immunocytochemistry revealed GFAP and MAO B expression. CONCLUSIONS: The expression of MAO B measured by 11C-DED uptake or immunocytochemistry was not significantly different in grade III or IV cells. The GFAP signal was higher for grade IV compared to grade III. 11C-MET uptake was high in all the tumor cells. 11C-DED is a dopamine analogue and the transport across cell membranes is expected to be mediated by DAT receptors present in astrocytes. Reactive astrocytes surround tumor lesions; so the authors suggest that the 11C-DED uptake might be caused by the reactive astrocytosis and not by MAO B expression in tumor cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Astrocitoma / Neoplasias Encefálicas / Astrocitos / Radiofármacos / Gliosis Límite: Humans Idioma: En Revista: Cancer Biother Radiopharm Asunto de la revista: FARMACIA / FARMACOLOGIA / NEOPLASIAS / TERAPEUTICA Año: 2017 Tipo del documento: Article País de afiliación: Uruguay Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Astrocitoma / Neoplasias Encefálicas / Astrocitos / Radiofármacos / Gliosis Límite: Humans Idioma: En Revista: Cancer Biother Radiopharm Asunto de la revista: FARMACIA / FARMACOLOGIA / NEOPLASIAS / TERAPEUTICA Año: 2017 Tipo del documento: Article País de afiliación: Uruguay Pais de publicación: Estados Unidos