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Rapid visual detection of lily mottle virus using a loop-mediated isothermal amplification method.
Zhao, Binan; Yang, Dan; Zhang, Yongchun; Xu, Yan; Zhao, Xiao; Liang, Jieling; Fan, Xiaorui; Du, Yanan; Zhu, Ziping; Shi, Bin; Zhang, Qi; Zhang, Xiaoxia; Cai, Youming; Zhao, Kai.
Afiliación
  • Zhao B; Department of Clinical Medicine, XuZhou Medical University, Xuzhou, People's Republic of China.
  • Yang D; School of Life science, Taizhou University, Taizhou, People's Republic of China.
  • Zhang Y; Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
  • Xu Y; College of Life and Environment Sciences, Shanghai Normal University, Shanghai, People's Republic of China.
  • Zhao X; Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
  • Liang J; Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
  • Fan X; School of Life science, Taizhou University, Taizhou, People's Republic of China.
  • Du Y; College of Life and Environment Sciences, Shanghai Normal University, Shanghai, People's Republic of China.
  • Zhu Z; College of Life and Environment Sciences, Shanghai Normal University, Shanghai, People's Republic of China.
  • Shi B; School of Life science, Taizhou University, Taizhou, People's Republic of China.
  • Zhang Q; Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
  • Zhang X; College of Life and Environment Sciences, Shanghai Normal University, Shanghai, People's Republic of China.
  • Cai Y; College of Life and Environment Sciences, Shanghai Normal University, Shanghai, People's Republic of China.
  • Zhao K; Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China. cym59059@163.com.
Arch Virol ; 163(2): 545-548, 2018 Feb.
Article en En | MEDLINE | ID: mdl-29134340
Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMoV, with no cross-reaction with other lily viruses. The sensitivity of LMoV using the LAMP assay was 100 times more sensitive than that using conventional polymerase chain reaction. A reverse transcription LAMP (RT-LAMP) was then successfully applied to detect LMoV RNA. The newly established LAMP and one-step RT-LAMP provide an alternative method for detecting LMoV in lily plants.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Potyvirus / Técnicas de Amplificación de Ácido Nucleico / Lilium Tipo de estudio: Diagnostic_studies / Evaluation_studies Idioma: En Revista: Arch Virol Año: 2018 Tipo del documento: Article Pais de publicación: Austria
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Potyvirus / Técnicas de Amplificación de Ácido Nucleico / Lilium Tipo de estudio: Diagnostic_studies / Evaluation_studies Idioma: En Revista: Arch Virol Año: 2018 Tipo del documento: Article Pais de publicación: Austria