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Prevalence of extended-spectrum beta-lactamase and carbapenemase-producing bloodstream isolates of Klebsiella pneumoniae in a tertiary care hospital.
Abodakpi, Henrietta; Chang, Kai-Tai; Sánchez Díaz, Ana María; Cantón, Rafael; Lasco, Todd M; Chan, Katrina; Sofjan, Amelia K; Tam, Vincent H.
Afiliación
  • Abodakpi H; a Department of Pharmacological and Pharmaceutical Sciences , University of Houston College of Pharmacy , Houston , TX , USA.
  • Chang KT; b Department of Pharmacy Practice and Translational Research , University of Houston College of Pharmacy , Houston , TX , USA.
  • Sánchez Díaz AM; c Servicio de Microbiología , Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS) , Madrid , Spain.
  • Cantón R; c Servicio de Microbiología , Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS) , Madrid , Spain.
  • Lasco TM; d Department of Pathology , Baylor St. Luke's Medical Center , Houston , TX , USA.
  • Chan K; a Department of Pharmacological and Pharmaceutical Sciences , University of Houston College of Pharmacy , Houston , TX , USA.
  • Sofjan AK; b Department of Pharmacy Practice and Translational Research , University of Houston College of Pharmacy , Houston , TX , USA.
  • Tam VH; a Department of Pharmacological and Pharmaceutical Sciences , University of Houston College of Pharmacy , Houston , TX , USA.
J Chemother ; 30(2): 115-119, 2018 Apr.
Article en En | MEDLINE | ID: mdl-29125052
To improve prescribing of empiric therapy, the local molecular epidemiology of extended-spectrum beta-lactamases (ESBLs) and Klebsiella pneumoniae carbapenemases (KPCs) in bloodstream isolates of K. pneumoniae were evaluated. Isolates resistant to third generation cephalosporins were screened phenotypically for ESBLs and carbapenemases, and subsequently confirmed by PCR for the presence of ESBL (blaTEM, blaSHV and blaCTX-M) and carbapenemase (blaKPC, blaVIM, blaNDM and blaOXA-48) genes. Hydrolytic activity (functional gene expression) was quantified using a nitrocefin degradation assay and correlated to ceftazidime or meropenem MIC. Clonality was assessed by repetitive element-based PCR. Beta-lactamases were functionally expressed in 13 isolates (15.5%); 7 (53.8%) harboured blaCTX-M-15 and 6 (46.2%) carried the blaKPC-2 gene. Correlation of hydrolytic activity to MIC yielded a coefficient of 98% for isolates expressing ESBLs alone and 56% for carbapenemase producers. Four unique ESBL-expressing clones and five carbapenem-resistant clones were identified. All 13 resistant isolates were susceptible to ceftazidime/avibactam (MIC ≤ 8/4 mg/L).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Beta-Lactamasas / Infecciones por Klebsiella / Farmacorresistencia Bacteriana / Klebsiella pneumoniae Tipo de estudio: Prevalence_studies / Risk_factors_studies Límite: Humans País/Región como asunto: America do norte Idioma: En Revista: J Chemother Asunto de la revista: ANTINEOPLASICOS / TERAPIA POR MEDICAMENTOS Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Beta-Lactamasas / Infecciones por Klebsiella / Farmacorresistencia Bacteriana / Klebsiella pneumoniae Tipo de estudio: Prevalence_studies / Risk_factors_studies Límite: Humans País/Región como asunto: America do norte Idioma: En Revista: J Chemother Asunto de la revista: ANTINEOPLASICOS / TERAPIA POR MEDICAMENTOS Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido