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Validated thin-layer chromatographic method for the identification and monitoring of the effect of the extraction method on the yield and phytochemical constituents of Egyptian Withania somnifera leaves.
Mahrous, Rahma S R; Fathy, Hoda M; Abu El-Khair, Rasha M; Omar, Abdallah A.
Afiliación
  • Mahrous RSR; Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
  • Fathy HM; Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
  • Abu El-Khair RM; Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
  • Omar AA; Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
J Sep Sci ; 41(2): 518-524, 2018 Jan.
Article en En | MEDLINE | ID: mdl-29115713
A sensitive, reliable, simple and rapid thin-layer chromatographic method has been developed for routine analysis of withanolide S content for the purpose of quality control assessment of chemotype III of Withania somnifera. The new method was used first to compare the accumulation of withanolide S in different parts of the plant, which was found to be the highest in the leaves extract (0.21% w/w). Second, to investigate different extraction parameters that improve the extraction efficiency of withanolides from the leaves using conventional and ultrasound-assisted extraction methods. The extraction efficiency was expressed via total withanolide content and withanolide S content.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatografía en Capa Delgada / Hojas de la Planta / Withania / Witanólidos Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: J Sep Sci Año: 2018 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatografía en Capa Delgada / Hojas de la Planta / Withania / Witanólidos Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: J Sep Sci Año: 2018 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Alemania