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Two tissue-specific factors bind the erythroid promoter of the human porphobilinogen deaminase gene.
Mignotte, V; Wall, L; deBoer, E; Grosveld, F; Romeo, P H.
Afiliación
  • Mignotte V; Laboratory of Gene Structure and Expression, National Institute for Medical Research, London, UK.
Nucleic Acids Res ; 17(1): 37-54, 1989 Jan 11.
Article en En | MEDLINE | ID: mdl-2911469
We have studied the erythroid-specific promoter of the human gene coding for Porphobilinogen Deaminase (PBGD) by DNaseI footprinting, gel retardation and methylation interference assays. We show that this promoter, which is inducible during MEL cell differentiation, contains three binding sites for the erythroid-specific factor NF-E1 and one site for a second erythroid-specific factor, which we name NF-E2. NF-E1 is a factor that also binds the promoter and the enhancer (present in the 3' flanking region) of the human beta-globin gene. NF-E2 has not yet been described and although it binds to a sequence containing the Ap1 consensus, it appears to be different from Ap1.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidroximetilbilano Sintasa / Regiones Promotoras Genéticas / Proteínas de Unión al ADN / Eritrocitos / Genes / Amoníaco-Liasas Límite: Animals / Humans Idioma: En Revista: Nucleic Acids Res Año: 1989 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidroximetilbilano Sintasa / Regiones Promotoras Genéticas / Proteínas de Unión al ADN / Eritrocitos / Genes / Amoníaco-Liasas Límite: Animals / Humans Idioma: En Revista: Nucleic Acids Res Año: 1989 Tipo del documento: Article Pais de publicación: Reino Unido