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Treatment with the MAPK kinase inhibitor U0126 during the first two hours of in vitro maturation improves bovine oocyte developmental competence.
Shinohara, T; Ohta, Y; Kawate, N; Takahashi, M; Sakagami, N; Inaba, T; Tamada, H.
Afiliación
  • Shinohara T; Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
  • Ohta Y; Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
  • Kawate N; Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
  • Takahashi M; Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Japan.
  • Sakagami N; Kanagawa Prefectural Livestock Industry Technology Center, Ebina, Kanagawa, Japan.
  • Inaba T; Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
  • Tamada H; Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
Reprod Domest Anim ; 53(1): 270-273, 2018 Feb.
Article en En | MEDLINE | ID: mdl-29110347
This study examined the effects of treatment with U0126, which inhibits MAPK by inhibiting MAPK kinase, during the first 2 hr of in vitro maturation on bovine developmental competence and on gap junction (GAPJ) communication between the oocyte and cumulus cells. The percentage of oocytes developing to the blastocyst stage in the group treated with 5 µM U0126 (28%) was significantly higher than that in controls (15%, p < .05), while that in the group treated with 10 µM U0126 (18%) was not. Breakdown of the GAPJs was delayed in the group treated with 5 µM U0126 when compared to controls, as estimated by immunohistochemical examination of connexin 43, which is a primary constituent of the GAPJs. These results indicate that treatment with 5 µM U0126 during in vitro maturation delays GAPJ breakdown and improves bovine oocyte developmental competence.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Butadienos / Bovinos / Quinasas de Proteína Quinasa Activadas por Mitógenos / Técnicas de Maduración In Vitro de los Oocitos / Nitrilos Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Butadienos / Bovinos / Quinasas de Proteína Quinasa Activadas por Mitógenos / Técnicas de Maduración In Vitro de los Oocitos / Nitrilos Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania