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Development of an automated wax-printed paper-based lateral flow device for alpha-fetoprotein enzyme-linked immunosorbent assay.
Preechakasedkit, Pattarachaya; Siangproh, Weena; Khongchareonporn, Nanthika; Ngamrojanavanich, Nattaya; Chailapakul, Orawon.
Afiliación
  • Preechakasedkit P; Program in Biotechnology, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
  • Siangproh W; Department of Chemistry, Faculty of Science, Srinakharinwirot University, Wattana, Bangkok 10110, Thailand.
  • Khongchareonporn N; The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
  • Ngamrojanavanich N; The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand; Electrochemistry and Optical Spectroscopy Center of Excellence (EOSCE), Department of Chemistry, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand. Elect
  • Chailapakul O; Electrochemistry and Optical Spectroscopy Center of Excellence (EOSCE), Department of Chemistry, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand; Center for Petroleum, Petrochemicals and Advanced Materials, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
Biosens Bioelectron ; 102: 27-32, 2018 Apr 15.
Article en En | MEDLINE | ID: mdl-29107857
In this study, a novel wax-printed paper-based lateral flow device has been developed as an alternative approach for an automated and one-step enzyme-linked immunosorbent assay (ELISA). The design pattern consisted of a non-delayed channel, a wax-delayed channel, a test zone and a control zone. This system was easily fabricated on a nitrocellulose membrane using a wax-printing method and then baked in an oven at 100°C for 1min. The four barriers of the wax-delayed channel could delay the flow time for 11s compared to the flow time of the non-delayed channel. To use the device under optimal conditions, alpha-fetoprotein (AFP) was detected at a limit of detection of 1ngmL-1 and assessed with the naked eye within 10min. A colorimetric intensity was also measured using a smart phone and computer software at a linear range of 0.1-100ngmL-1 with a good correlation. Furthermore, the proposed device was successfully applied to detect AFP in human serum. Therefore, the wax-printing demonstrates a user-friendly, easy and quick method for the fabrication of the device, which could be used as a one-step, portable, disposable, low-cost, simple, instrument-free and point-of-care device for the automated ELISA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papel / Ensayo de Inmunoadsorción Enzimática / Técnicas Biosensibles / Alfa-Fetoproteínas / Colodión / Técnicas Analíticas Microfluídicas Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Humans Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papel / Ensayo de Inmunoadsorción Enzimática / Técnicas Biosensibles / Alfa-Fetoproteínas / Colodión / Técnicas Analíticas Microfluídicas Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Humans Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Reino Unido