Your browser doesn't support javascript.
loading
miR-324-3p promotes gastric cancer development by activating Smad4-mediated Wnt/beta-catenin signaling pathway.
Sun, Guang-Li; Li, Zheng; Wang, Wei-Zhi; Chen, Zheng; Zhang, Lei; Li, Qing; Wei, Song; Li, Bo-Wen; Xu, Jiang-Hao; Chen, Liang; He, Zhong-Yuan; Ying, Kai; Zhang, Xuan; Xu, Hao; Zhang, Dian-Cai; Xu, Ze-Kuan.
Afiliación
  • Sun GL; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • Li Z; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • Wang WZ; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • Chen Z; Department of Surgery, Vanderbilt University Medical Center, Nashville, TN, USA.
  • Zhang L; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • Li Q; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • Wei S; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • Li BW; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • Xu JH; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • Chen L; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • He ZY; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • Ying K; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • Zhang X; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • Xu H; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • Zhang DC; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China.
  • Xu ZK; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou road, Nanjing, Jiangsu, China. xuzekuan@njmu.edu.cn.
J Gastroenterol ; 53(6): 725-739, 2018 Jun.
Article en En | MEDLINE | ID: mdl-29103082
BACKGROUND: Emerging evidence suggested that miRNAs can function as oncogenes or tumor suppressors by regulating downstream target genes. miR-324-3p has been reported to function in several carcinomas, but its role in gastric cancer (GC) is still unknown. This study aims to explore the effects of miR-324-3p on the development of GC. METHODS: Expression of miR-324-3p was examined in GC cells and tissues by qRT-PCR. Effects of miR-324-3p on GC cells were evaluated by cell vitality assay, colony formation assay, cell migration assay, and flow cytometric assay. The dual luciferase assay was used to verify whether miR-324-3p could interact with the potential target genes. Western blot was used to assess the expression level of Smad4 and beta-catenin. Intracellular ATP level was also examined. The tumor xenografts were established using nude mice. A gastric organoid model was made from fresh stomach tissue. RESULTS: miR-324-3p was expressed at higher levels in the tumor tissues compared with adjacent normal tissues. Overexpression of miR-324-3p promoted cell growth, migration, and decreased apoptosis. miR-324-3p repressed the expression of Smad4, and loss of Smad4 activated the Wnt/beta-catenin signaling pathway. Overexpression of Smad4 rescued the effects of miR-324-3p on GC cells. The intracellular ATP level was upregulated with overexpression of miR-324-3p. miR-324-3p facilitated tumor cell colonization and growth in vivo and contributed to the growth of gastric organoids. CONCLUSIONS: The results suggested that miR-324-3p promoted GC through activating the Smad4-mediated Wnt/beta-catenin signaling pathway. The miR-324-3p/Smad4/Wnt signaling axis may be a potential therapeutic target to prevent GC progression.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Gástricas / MicroARNs / Proteína Smad4 / Vía de Señalización Wnt Límite: Adult / Aged / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: J Gastroenterol Asunto de la revista: GASTROENTEROLOGIA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Japón
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Gástricas / MicroARNs / Proteína Smad4 / Vía de Señalización Wnt Límite: Adult / Aged / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: J Gastroenterol Asunto de la revista: GASTROENTEROLOGIA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Japón