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A Red Algal Bourbonane Sesquiterpene Synthase Defined by Microgram-Scale NMR-Coupled Crystalline Sponge X-ray Diffraction Analysis.
Kersten, Roland D; Lee, Shoukou; Fujita, Daishi; Pluskal, Tomás; Kram, Susan; Smith, Jennifer E; Iwai, Takahiro; Noel, Joseph P; Fujita, Makoto; Weng, Jing-Ke.
Afiliación
  • Kersten RD; Whitehead Institute for Biomedical Research , 455 Main Street, Cambridge, Massachusetts 02142, United States.
  • Lee S; Graduate School of Engineering, The University of Tokyo, JST-ACCEL , Tokyo 113-8654, Japan.
  • Fujita D; Whitehead Institute for Biomedical Research , 455 Main Street, Cambridge, Massachusetts 02142, United States.
  • Pluskal T; Graduate School of Engineering, The University of Tokyo, JST-ACCEL , Tokyo 113-8654, Japan.
  • Kram S; Whitehead Institute for Biomedical Research , 455 Main Street, Cambridge, Massachusetts 02142, United States.
  • Smith JE; Scripps Institution of Oceanography, University of California, San Diego , La Jolla, California 92093, United States.
  • Iwai T; Scripps Institution of Oceanography, University of California, San Diego , La Jolla, California 92093, United States.
  • Noel JP; Graduate School of Engineering, The University of Tokyo, JST-ACCEL , Tokyo 113-8654, Japan.
  • Fujita M; Howard Hughes Medical Institute, Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies , La Jolla, California 92037, United States.
  • Weng JK; Graduate School of Engineering, The University of Tokyo, JST-ACCEL , Tokyo 113-8654, Japan.
J Am Chem Soc ; 139(46): 16838-16844, 2017 11 22.
Article en En | MEDLINE | ID: mdl-29083151
Sesquiterpene scaffolds are the core backbones of many medicinally and industrially important natural products. A plethora of sesquiterpene synthases, widely present in bacteria, fungi, and plants, catalyze the formation of these intricate structures often with multiple stereocenters starting from linear farnesyl diphosphate substrates. Recent advances in next-generation sequencing and metabolomics technologies have greatly facilitated gene discovery for sesquiterpene synthases. However, a major bottleneck limits biochemical characterization of recombinant sesquiterpene synthases: the absolute structural elucidation of the derived sesquiterpene products. Here, we report the identification and biochemical characterization of LphTPS-A, a sesquiterpene synthase from the red macroalga Laurencia pacifica. Using the combination of transcriptomics, sesquiterpene synthase expression in yeast, and microgram-scale nuclear magnetic resonance-coupled crystalline sponge X-ray diffraction analysis, we resolved the absolute stereochemistry of prespatane, the major sesquiterpene product of LphTPS-A, and thereby functionally define LphTPS-A as the first bourbonane-producing sesquiterpene synthase and the first biochemically characterized sesquiterpene synthase from red algae. Our study showcases a workflow integrating multiomics approaches, synthetic biology, and the crystalline sponge method, which is generally applicable for uncovering new terpene chemistry and biochemistry from source-limited living organisms.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Am Chem Soc Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Am Chem Soc Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos